WANG Ye, ZHU Xiang-dong. Effect of Gegen Qinlian Tang on CRP, TNF-alpha and IL-6 in Diabetic ZDF Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 130-134.
DOI:
WANG Ye, ZHU Xiang-dong. Effect of Gegen Qinlian Tang on CRP, TNF-alpha and IL-6 in Diabetic ZDF Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 130-134. DOI: 10.13422/j.cnki.syfjx.2017210130.
Effect of Gegen Qinlian Tang on CRP, TNF-alpha and IL-6 in Diabetic ZDF Rats
Objective: To investigate the effects of Gegen Qinlian Tang (GQT) on expressions of inflammatory cytokines C reactive protein (CRP)
tumor necrosis factor-α (TNF-α)
interleukin-6 (IL-6) of Zucker diabetes fatty (ZDF) rats with type 2 diabetes mellitus (T2DM). Method: The 8 male ZDF(fa/+) rats were used as normal group
24 male ZDF rats (fa/fa) rats were randomized divided into model group (Nacl
10 mL·kg-1)
Metformin group (300 mg·kg-1)
GQT group (13.8 g·kg-1); and Zucker lean rats (ZL) were included into the blank control group
with 8 rats in each group. Weight and fasting blood-glucose(FBG) of rats were observed once every two weeks. Samples were taken 8 weeks after administration. Automatic biochemical analyzer was used to triglyceride(TG) and total cholesterol(TC). Enzyme linked immunosorbent assay(ELISA) was used to detect CRP and fasting insulin in rat serum(FINS)
and the expressions of TNF-α and IL-6 was detected by Western blot. Result: Compared with model group
GQT group show significant improvements in weight
FBG
TG
TC
CRP
FINS
IRI and ISI (P < 0.05). Meanwhile
GQT group can reduce the expressions of CRP
TNF-α and IL-6 in obesity type T2DM rats (P < 0.05). Conclusion: GQT can effectively reduce insulin resistance among ZDF rats with T2DM. Its action mechanism may be correlated with decrease in expressions of cytokines in relevant low-grade inflammatory reactions.
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Related Institution
Nanyang Medical College
Dongzhimen Hospital, Beijing University of Chinese Medicine
Tianjin Academy of Traditional Chinese Medicine(TCM) Affiliated Hospital
School of Chinese Materia Medica, Tianjin University of TCM
Guangdong Provincial Engineering Laboratory of Chinese Medicine Preparation Technology, Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, School of Traditional Chinese Medicine, Southern Medical University