Correlation Analysis Between Contents of Active Ingredients and Grades of Asparagi Radix

CAO Xu-lin; XU Chang-yan; DENG Qiang; FAN Ju-di; QIN Rong-gui

doi:10.13422/j.cnki.syfjx.2017220055

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Correlation Analysis Between Contents of Active Ingredients and Grades of Asparagi Radix

更新时间：2024-01-04

- Correlation Analysis Between Contents of Active Ingredients and Grades of Asparagi Radix
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 22, Pages: 55-59(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017220055
  CLC： R284.1
- Published：2017
- 稿件说明：
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CAO Xu-lin, XU Chang-yan, DENG Qiang, et al. Correlation Analysis Between Contents of Active Ingredients and Grades of Asparagi Radix[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 55-59.
DOI：

CAO Xu-lin, XU Chang-yan, DENG Qiang, et al. Correlation Analysis Between Contents of Active Ingredients and Grades of Asparagi Radix[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 55-59. DOI： 10.13422/j.cnki.syfjx.2017220055.

摘要

目的：测定不同等级天冬药材中醇溶性浸出物和活性物质总皂苷、总多糖的含量，分析天冬商品等级与醇溶性浸出物、活性物质含量之间的相关性，考察商品天冬等级划分方法的合理性。方法：根据2015年版《中国药典》测定不同商品等级天冬药材醇溶性浸出物含量；采用紫外-可见分光光度法测定37份不同等级天冬中总皂苷含量，检测波长408 nm；采用苯酚-硫酸法测定总多糖含量，检测波长490 nm；运用相关分析和线性回归分析天冬药材醇溶性浸出物、总皂苷及总多糖含量与商品等级间的相关性。结果：不同等级天冬药材醇溶性浸出物和总皂苷含量有显著性差异（P<0.05），一等品与三等品比较天冬总多糖含量具显著性差异（P<0.05），其余各等级间总多糖含量差异无显著性。在相关性分析中，天冬商品等级与醇溶性浸出物、总皂苷含量呈极显著正相关关系（P<0.01），与总多糖含量呈显著正相关关系（P<0.05）。结论：商品天冬以药材中部直径为主要标准的分级方法能体现天冬醇溶性浸出物和内在活性物质总皂苷含量，传统等级划分方法仍具有合理性；天冬药材建议以测定总皂苷含量为主，醇溶性浸出物和总多糖含量作为辅助质量评价指标。

Abstract

Objective: To determine the contents of alcohol-soluble extractive

total saponins and total polysaccharides in different grades of Asparagi Radix

and to explore the correlation between the effective components and their grades for investigating the rationality of grading method of Asparagi Radix. Method: The content of alcohol-soluble extraction of Asparagi Radix with different grade was determined according to the 2015 edition of Chinese Pharmaeopoeia.Ultraviolet visible spectrophotometry was used to determine the content of total saponins in Asparagi Radix samples with detection wavelength at 408 nm.The content of total polysaccharides was quantified by phenol-sulfuric acid method.The correlation analysis and linear regression analysis were used to analyze the correlation between the grades and quantitative indexes. Result: The contents of alcohol-soluble extractive and total saponins existed significant difference (P<0.05) in different grades of Asparagi Radix. The content of total polysaccharides was significantly different (P<0.05) between the first and the third grade

but the difference was not significant between other grades.The correlation analysis indicated that the grades showed extremely significant positive correlation with alcohol-soluble extractive content and total saponins content(P<0.01)

and showed significant positive correlation with total polysaccharides content(P<0.05). Conclusion: The contents of total saponins and alcohol-soluble extractive in Asparagi Radix are consistent with the classification method by taking diameter of the middle part of medicine as the main standard

which indicates that the grading method of Asparagi Radix is still reasonable.Quality evaluation of Asparagi Radix is recommended to determine the content of total saponins by taking contents of alcohol-soluble extractive and total polysaccharides as the secondary indicators.

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