Rapid Analysis of Chemical Constituents in Xuanmai Ganjie Granules by UPLC-Q-TOF-MS

WU Yin; QIU Zhi-hong; BAI Wan-jun; DONG Zhan-jun

doi:10.13422/j.cnki.syfjx.2017220070

您当前的位置：

首页 >

文章列表页 >

Rapid Analysis of Chemical Constituents in Xuanmai Ganjie Granules by UPLC-Q-TOF-MS

更新时间：2024-01-04

- Rapid Analysis of Chemical Constituents in Xuanmai Ganjie Granules by UPLC-Q-TOF-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 22, Pages: 70-76(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017220070
  CLC： R286.0
- Published：2017
- 稿件说明：
移动端阅览
WU Yin, QIU Zhi-hong, BAI Wan-jun, et al. Rapid Analysis of Chemical Constituents in Xuanmai Ganjie Granules by UPLC-Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 70-76.
DOI：

WU Yin, QIU Zhi-hong, BAI Wan-jun, et al. Rapid Analysis of Chemical Constituents in Xuanmai Ganjie Granules by UPLC-Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 70-76. DOI： 10.13422/j.cnki.syfjx.2017220070.

摘要

目的：采用超高效液相色谱串联四级杆飞行时间质谱（UPLC-Q-TOF-MS）技术对玄麦甘桔颗粒的化学成分进行检测分析。方法：采用 XB-Bridge BEH C18色谱柱（2.1 mm×100 mm，2.5 μm），以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱，流速0.3 mL ·min-1。质谱使用 ESI 离子源，正、负离子模式扫描采集数据。结果：根据精确相对分子质量和碎片离子分析，并结合相关文献检索及质谱裂解规律，共鉴定出 33 个化学成分，9 个环烯醚萜类成分，20个黄酮类成分，4个皂苷类成分。结论：该文建立了一种基于UPLC-Q-TOF-MS 的快速成分分析方法，为研究玄麦甘桔颗粒的化学成分，及进一步物质基础研究提供了技术支持。

Abstract

Objective: To analyze and identify the chemical constituents of Xuanmai Ganjie granules by ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Method: The separation was performed on an XB-Bridge BEH C18 column (2.1 mm×100 mm

2.5 μm) with acetonitrile-0.1% formic acid solution as mobile phase for gradient elution. The flow rate was 0.3 mL · min-1. Electrospray ionization source was used to scan and collect data in positive and negative ion modes. Result: According to the high resolution MS spectra data

fragmentation ion information and retention time

33 chemical constituents of Xuanmai Ganjie granules were identified including 9 iridoid glycosides

20 flavonoids

and 4 saponins by comparison with literature reports and MS cleavage mechanism. Conclusion: The established rapid analysis method based on UPLC-Q-TOF-MS could provide technical support for studying the chemical constituents of Xuanmai Ganjie granules and further studies on its material basis.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Ultrasonic-assisted Extraction of Squalene from Glycyrrhizae Radix Et Rhizoma and Analysis of Crude Fat Components

Identification of Chemical Constituents of Bidens pilosa and Analysis of Its Anti-gastric Cancer Cell Proliferation Activity in Vitro

Chemical Constituents， Pharmacological Activities， and Identification of Amomi Fructus： A Review

Analysis of Material Basis of Famous Classical Formula Baoyuantang Based on UPLC-Q-TOF-MS/MS

Structure-activity Omics on Anti-inflammatory and Analgesic Effect of Glycyrrhizae Radix et Rhizoma in Qizhiweitong Granules

Related Author

GAO Gui-tao

YANG Min-li

LIANG Xin-hua

HAN Yu

LIU Chang

LIU Jiao

ZHANG Tao

ZOU Zhongmei

Related Institution

Shenyang Pharmaceutical University

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences&Peking Union College

Henan University of Chinese Medicine

School of Food Science and Engineering，Guangdong Ocean University

School of Materials Science and Engineering，Guangdong Ocean University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰