Effect of Jianpi Yichang Powder on Expression Levels of HSP70 in Colon Tissues of Rats with Ulcerative Colitis

LIN Xiao-yuan; YU Xing; LEI Gui-yue; LI Zhi-yuan; GU Juan; WANG Min; LIU Jie-min

doi:10.13422/j.cnki.syfjx.2017220091

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Effect of Jianpi Yichang Powder on Expression Levels of HSP70 in Colon Tissues of Rats with Ulcerative Colitis

更新时间：2024-01-04

- Effect of Jianpi Yichang Powder on Expression Levels of HSP70 in Colon Tissues of Rats with Ulcerative Colitis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 22, Pages: 91-96(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017220091
  CLC： R285.5
- Published：2017
- 稿件说明：
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LIN Xiao-yuan, YU Xing, LEI Gui-yue, et al. Effect of Jianpi Yichang Powder on Expression Levels of HSP70 in Colon Tissues of Rats with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 91-96.
DOI：

LIN Xiao-yuan, YU Xing, LEI Gui-yue, et al. Effect of Jianpi Yichang Powder on Expression Levels of HSP70 in Colon Tissues of Rats with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 91-96. DOI： 10.13422/j.cnki.syfjx.2017220091.

摘要

目的：探讨健脾益肠散对溃疡性结肠炎（UC）大鼠结肠组织热休克蛋白70（HSP70）蛋白和mRNA表达的影响。方法：将健康SPF级雄性SD大鼠60只，随机分为2组，正常组和造模组；造模组采用二硝基苯磺酸（TNBS）/乙醇法复制UC大鼠模型；待复制模型成功后将造模组随机分为5组，分别为模型组、柳氮磺吡啶组（0.3 g ·kg-1）以及健脾益肠散高、中、低剂量组（204，136，68 g ·kg-1），每组10只；灌胃相应药物21 d后，观察各组大鼠的一般状态和结肠黏膜组织损伤情况，免疫组化、蛋白免疫印迹法（Western blot）和实时荧光定量 PCR（Real-time PCR）分别检测大鼠结肠组织中HSP70的蛋白和mRNA表达。结果：与正常组比较，模型组大鼠结肠黏膜损伤评分显著升高（P<0.01），HSP70蛋白和mRNA表达均显著降低（P<0.01）。与模型组比较，各给药组结肠黏膜损伤评分均显著降低（P<0.01），各给药组均可增加结肠组织HSP70的蛋白和mRNA表达（P<0.05，P<0.01），其中以健脾益肠散高剂量组最为明显（P<0.05，P<0.01）。结论：健脾益肠散可能通过促进HSP70的表达而达到对UC大鼠结肠黏膜的免疫保护，从而发挥治疗作用。

Abstract

Objective: To explore the effect of Jianpi Yichang powder (JP) on protein and mRNA expression levels of heat shock protein 70 (HSP70) in colon tissues of rats with ulcerative colitis (UC). Method: The 60 healthy SPF male SD rats were randomly divided into normal group and model group. The UC models were established by trinitro-benzene-sulfonic acid(TNBS)/ethanol method. Then the successfully modeled rats were randomly divided into the model group

sulfasalazine group (0.3 g ·kg-1)

and JP high

middle and low dose groups (204

136 and 68 g ·kg-1) with 10 rats in each group. After ig administration for 21 days

the general condition and the colonic mucosa injury of rats were observed. Immunohistochemistry

Western blot and Real-time PCR were used to detect the protein and mRNA expression levels of HSP70 in colon tissues of rats. Result: As compared with the normal group

the colon mucosal injury score was increased significantly (P<0.01)

and the protein and mRNA expression levels of HSP70 were significantly decreased (P<0.01) in model group. As compared with the model group

the colon mucosal injury scores in each drug group were significantly lower (P<0.01)

and the protein and mRNA expression levels of HSP70 were significantly increased (P<0.05

P<0.01). The effect was most obvious in JP high dose group (P<0.05

P<0.01). Conclusion: JP may promote the expression of HSP70 to protect intestinal mucosal immunity in UC rats

so as to play a therapeutic role.

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