Effect of Xuefu Zhuyu Capsule in Regulating Jagged1/Notch Signal in Angiogenesis

WANG Yi-zheng; LIN Fan; CAI Fei; HU Ya-qiong; GAO Dong

doi:10.13422/j.cnki.syfjx.2017220132

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Effect of Xuefu Zhuyu Capsule in Regulating Jagged1/Notch Signal in Angiogenesis

更新时间：2024-01-04

- Effect of Xuefu Zhuyu Capsule in Regulating Jagged1/Notch Signal in Angiogenesis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 22, Pages: 132-136(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017220132
  CLC： R285
- Published：2017
- 稿件说明：
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WANG Yi-zheng, LIN Fan, CAI Fei, et al. Effect of Xuefu Zhuyu Capsule in Regulating Jagged1/Notch Signal in Angiogenesis[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 132-136.
DOI：

WANG Yi-zheng, LIN Fan, CAI Fei, et al. Effect of Xuefu Zhuyu Capsule in Regulating Jagged1/Notch Signal in Angiogenesis[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 132-136. DOI： 10.13422/j.cnki.syfjx.2017220132.

摘要

目的：探讨Jagged1/Notch信号通路在血府逐瘀胶囊调控血管新生中的作用。方法：以2.5%的血府逐瘀胶囊含药血清和空白血清分别干预人微血管内皮细胞（HMEC-1），通过实时荧光定量PCR（Real-time PCR）检测药物处理细胞12，24，36，48 h时Jagged1 mRNA水平的表达，通过蛋白免疫印迹法（Western blot）检测药物处理细胞12，24，48 h时Jagged1在蛋白质水平的表达；在血府逐瘀胶囊促血管新生最佳药效条件下，使用γ-分泌酶抑制剂（3，5-二氟苯乙酰基）-L-丙氨酰基-L-2-苯基甘氨酸叔丁酯（DAPT）阻断Notch信号通路，设置DAPT处理组，二甲基亚砜（DMSO）溶剂组和空白组，通过体外成血管实验检测抑制Jagged1/Notch信号对药物诱导HMEC-1体外成血管能力的影响。结果：血府逐瘀胶囊含药血清干预内皮细胞12 h能上调Jagged1在mRNA水平表达（P<0.01），药物干预细胞24 h能同时上调Jagged1在mRNA和蛋白水平表达（P<0.05）；DAPT阻断Notch信号后，药物促体外成血管能力下降。结论：血府逐瘀胶囊可通过调控Jagged1表达，影响Jagged1/Notch信号通路，从而调控血管新生。

Abstract

Objective: To explore the effect of Xuefu Zhuyu capsule (XZC) in regulating Jagged1/Notch signal in angiogenesis. Method: XZC-containing serum and blank serum were used respectively to treat human microvascular endothelial cell-1 (HMEC-1). The mRNA expression of Jagged1 was detected by Real-time quantitative (PCR) at 12

48 h

and the protein expression of Jagged1 was detected by Western blot at 12

48 h. The γ-secretase inhibitor DAPT was used to block the Notch signaling pathway under the optimal condition of promoting angiogesis by XZC. The cells were randomly divided into 3 groups

namely DAPT processing group

solvent control group and blank control group. The angiogenesis effect was tested by an in vitro angiogenesis assay. Result: XZC up-regulated the mRNA expression of Jagged1 after culturing HMEC-1 cells for 12 h (P<0.01)

and the mRNA and protein expressions of Jagged1 were up-regulated at 24 h (P<0.05). The ability of angiogenesis in vitro was inhibited after the Notch signaling pathway was blocked by DAPT. Conclusion: XZC can regulate the expression of Jagged1

and Jagged1/Notch signaling pathway may be correlated with XZC's effect on regulating angiogenesis.

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