Preparation and Properties of Montmorillonite-betaxolol Hydrochloride Liposomes

LI Juan; HOU Dong-zhi; HU Sheng; HUANG Yi; TIAN Shuang-yan; CHEN Yan-zhong; LYU Zhu-fen; DUAN Hao-yun; ZHOU Qing-jun

doi:10.13422/j.cnki.syfjx.2017230007

您当前的位置：

首页 >

文章列表页 >

Preparation and Properties of Montmorillonite-betaxolol Hydrochloride Liposomes

更新时间：2024-01-04

- Preparation and Properties of Montmorillonite-betaxolol Hydrochloride Liposomes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 23, Pages: 7-12(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017230007
  CLC： R943
- Published：2017
- 稿件说明：
移动端阅览
LI Juan, HOU Dong-zhi, HU Sheng, et al. Preparation and Properties of Montmorillonite-betaxolol Hydrochloride Liposomes[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(23): 7-12.
DOI：

LI Juan, HOU Dong-zhi, HU Sheng, et al. Preparation and Properties of Montmorillonite-betaxolol Hydrochloride Liposomes[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(23): 7-12. DOI： 10.13422/j.cnki.syfjx.2017230007.

摘要

目的：优化盐酸倍他洛尔-蒙脱石脂质体（Mt-BH-LP）的处方及制备工艺，考察其体外释放性能和渗透性能。方法：采用乙醇注入法-硫酸铵梯度法制备Mt-BH-LP；以包封率和载药量为评价指标，采用正交试验优化Mt-BH-LP的处方；通过透析法考察Mt-BH-LP的体外释放性能；以家兔离体角膜为模型，采用体外改良的Franz扩散池法研究Mt-BH-LP的渗透性，以人永生化角膜上皮细胞（iHCEC）为模型，研究Mt-BH-LP的渗透性。结果：Mt-BH-LP处方工艺条件为卵磷脂-BH（5∶1），卵磷脂-胆固醇（9∶1），硫酸铵浓度0.15 mol·L-1，卵磷脂质量225 mg。Mt-BH-LP包封率（75.85±2.15）%，载药量（11.41±0.29）%，平均粒径（218±22.32） nm，Zeta电位（17.03±0.25） mV；体外释放试验表明Mt-BH-LP在10 h累计释放度达到60.2%，离体角膜透过试验结果表明Mt-BH-LP的角膜水化值[（76.72±2.68）%]在正常范围内。结论：采用乙醇注入法-硫酸铵梯度法制备的Mt-BH-LP包封率和载药量较高，缓释性能良好，具有良好的开发与应用前景。

Abstract

Objective: To optimize formulation and preparation technology of montmorillonite-betaxolol hydrochloride liposomes (Mt-BH-LP)

and evaluate its in vitro release performance and permeability. Method: Mt-BH-LP was prepared by ethanol injection combined with ammonium sulfate gradient method.With composite score of encapsulation efficiency and drug loading as index

Orthogonal test was used to optimize the formulation.The in vitro release of Mt-BH-LP was detected by dialysis method.With isolated corneas of rabbit as a model

the in vitro modified Franz diffusion cell method was used to measure the permeability of Mt-BH-LP.The permeability of Mt-BH-LP was further studied by taking human immortalized corneal epithelial cells (iHCEC) as model. Result: Optimum prescription was as following:ammonium sulfate concentration of 0.15 moL·L-1

lecithin quality of 225 mg

lecithin-BH (5:1)

lecithin-cholesterol (9:1).Encapsulation efficiency of Mt-BH-LP was (75.85±2.15)%

its drug loading

average particle size and Zeta potential were (11.41±0.29)%

(218±22.32) nm and (17.03±0.25) mV

respectively.The cumulative release of Mt-BH-LP was 60.2% in 10 h

the corneal hydration value of Mt-BH-LP was (76.72±2.68)% within the normal range. Conclusion: Mt-BH-LP prepared by optimized formulation has high encapsulation efficiency and drug loading

which displays good sustained-release performance

and it has a good development of application prospect.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Preparation and Release Behavior of Berberine Hydrochloride Liposomes

Preparation of Betaxolol Hydrochloride Loading Montmorillonite-chitosan Nanoparticles and Evaluation of Its Biological Properties

Determination of the Entrapment Efficiency and Drug Loading Capacity of Two Kinds of Biochanin A Loaded Lipid Nanoparticles

Determination of Entrapment Efficiency of Oleanolic Acid Liposomes

Preparation and Release Behavior Investigation of Ursolic Acid Liposome

Related Author

WANG Xing-hui

LI Jun-sheng

LV Jia-jia

LIU Zhi-hui

HOU Dong-zhi

GUI Ru-yi

WANG Qiang

CHENG Hai-lin

Related Institution

School of Pharmacy，Guangxi University of Chinese Medicine， South China Branch of National Engineering Research Center for Manufacturing Technology of Traditional Chinese Medicine Solid Preparation

School of Chinese Materia Medica， Tianjin University of Traditional Chinese Medicine

School of Pharmacy， Jiangxi University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Key Laboratory of Modern Preparation of Traditional Chinese Medicine （TCM）， Ministry of Education，Experimental Animal Science and Technology Center， Jiangxi University of TCM

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰