Objective: To establish the HPLC fingerprint chromatogram of Pericampylus glaucus for the quality control of multi-batches of medicinal material

and provide scientific basis for the research on quality standards of P. glaucus. Method: The separation was developed on C18 column (4.6 mm×250 mm

5 μm) with acetonitrile-0.2% phosphoric acid as the mobile phase for gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 327 nm and column temperature was 30℃. The HPLC fingerprint chromatogram of P. glaucus was set up

and the quality of 11 batches of P. glaucus was evaluated by similarity assay (Similarity evaluation system for chromatographic fingerprint of TCM

Version 2012.1) as well as principal component analysis and cluster analysis. Result: Eleven common peaks were marked in 10 batches of samples by using multi-point correction and Mark peak matching

with the fingerprint of S4 sample as the reference fingerprint; and chlorogenic acid was identified as compared with the control product. The similarity of fingerprint was all higher than 0.9.Samples from different producing areas were classified into four groups based on principal component cluster analysis. Conclusion: This method is highly stable

simple and reproducible. With stable fingerprint

this study has important significance on further research of P. glaucus pharmacodynamic material basis

and also provides a reference for the quality control and evaluation of Guangxi Zhuang medicine P. glaucus.