Objective: To recognize the main effective antifungal components against Candida albicans in Artemisia selengensis leaves. Method: The soluble components in water extract of A. selengensis leaves were preliminarily detected and analyzed

and then separated by HPD600 macroporous resin. Based on the knock-out/knock-in model

30% ethanol

50% ethanol

and 70% ethanol water solution were used to obtain different components and with their corresponding negative samples as the research object. Then the anti-fungal components in A. selengensis leaves were recognized by microdilution method

and HPLC-MS was combined for preliminary components analysis. Result: The contents of sugar

protein and other nutrients in water soluble components from water extract of A. selengensis accounted for about 78%

and polyphenols accounted for about 16%. The fraction C (70% ethanol elution) from A. selengensis leaves via HPD600 macroporous resin purification was the main anti-fungal component against C. albicans in a dose-effect dependence manner. Mass spectrometry analysis showed that fraction C was mainly of diterpenoid tanshinones and nalidixic acid compounds

including dehydrogenated tanshinone ⅡA

tanshinone ⅡA and tanshinone Ⅰ

as well as 3 kinds of nalidixic acid derivatives. The above substances were discovered from the A. selengensis for the first time. Conclusion: The main effective anti-fungal components against C. albicans in A. selengensis leaves were studied by constituents knock-out/knock-in strategy

providing the theoretical basis for the further study of monomeric compounds with antimicrobial activity from A. selengensis leaves extract.