Objective: To establish the HPLC fingerprint of Xinsuning capsules. Method: HPLC was employed

Welch Xtimate C18 column(4.6 mm×250 mm

5 μm) was used with acetonitrile-0.2% formic acid solution (containing 0.2% ammonium hydroxide) as the mobile phase in gradient elution.The flow rate was set at 1.0 mL·min-1 and the detection wavelength was set at 254 nm.Ten batches Xinsuning capsules were collected to assess the similarities

the main peaks in the fingerprint were identified by comparing the retention time of reference substances

and the source and attribution of chemical components in this preparation were analyzed by comparing with single herb. Result: The established fingerprint of Xinsuning capsules was was sensitive and specific.There were 29 common peaks in different batches of Xinsuning capsules and 12 peaks were identified.Compared with the reference fingerprint

the fingerprint similarities of ten batches of Xinsuning capsules were more than 0.98.A comparative analysis showed the contribution of Coptidis Rhizoma and Aurantii Fructus Immaturus was the highest

their contribution rates were 0.21. Conclusion: The analytical method of HPLC fingerprint is stable and reliable with fine repeatability

which provides a reference for study on material basis and quality control of Xinsuning capsules.