Analysis of Metabolites of  Extract in Rat Feces

GONG Zi-peng; WU Lin-lin; WU Ping; LI Mei; HOU Jing-yu; CHEN Ting-ting; LAN Yan-yu; LI Yong-jun; LI Yue-ting; WANG Yong-lin

doi:10.13422/j.cnki.syfjx.2017240100

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Analysis of Metabolites of Extract in Rat Feces

更新时间：2024-01-04

- Analysis of Metabolites of Extract in Rat Feces
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 24, Pages: 100-105(2017)
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- DOI：10.13422/j.cnki.syfjx.2017240100
  CLC： R285.5
- Published：2017
- 稿件说明：
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GONG Zi-peng, WU Lin-lin, WU Ping, et al. Analysis of Metabolites of Extract in Rat Feces[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(24): 100-105.
DOI：

GONG Zi-peng, WU Lin-lin, WU Ping, et al. Analysis of Metabolites of Extract in Rat Feces[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(24): 100-105. DOI： 10.13422/j.cnki.syfjx.2017240100.

摘要

目的：研究羊耳菊提取物给药后在大鼠粪便中的代谢产物，为该药材的后续研究与开发提供参考。方法：采用超高效液相色谱-三重四级杆飞行时间质谱（UPLC-Q-TOF-MS/MS）检测大鼠口服羊耳菊提取物后粪便中的代谢产物，每次给药剂量100 g·kg-1。使用RRHD Eclipse Plus C18色谱柱（2.1 mm×100 mm，1.8 μm），流动相0.1%甲酸水溶液-0.1%甲酸乙腈溶液梯度洗脱，选择电喷雾离子源，负离子模式进行扫描，准确质量测定采用甲酸钠校正标准液，采用Metabolite Detect （micrOTOF 2.3）等软件进行数据处理，鉴定代谢产物的化学结构。结果：在大鼠粪便中检测到原型M8（1，3-O-二咖啡酰基奎宁酸），M1~M4（二氢单咖啡酰基奎宁酸异构化产物），M5（二氢咖啡酸的硫酸酯化产物），M6~M7（单咖啡酰基奎宁酸的甲基化产物）等22个代谢产物。结论：羊耳菊提取物给药后，咖啡酰基奎宁酸类活性成分在大鼠粪便中的代谢途径以甲基化、还原反应为主。

Abstract

Objective: To investigate the metabolites of Inula cappa extract in rat feces after oral administration. Method: UPLC-Q-TOF-MS/MS was used for analyzing the metabolites of I. cappa extract in rat feces after oral administration at a dose of 100 g·kg-1.Separation of the metabolites was performed on an RRHD Eclipse Plus C18 column (2.1 mm×100 mm

1.8 μm) with the mobile phase of 0.1% formic acid-0.1% formic acid acetonitrile solution for gradient elution

and the samples were treated by electron spray negative ionization mode (ESI-).Then the data were processed with metabolite tools softwares to identify the chemical structure of metabolites. Result: M8 (1

3-O-dicaffeoylquinic acid) and 21 other metabolites were identified

including M1-M4(hydrogenation of caffeoylquinic acid)

M5(sulfation of dihydrocaffeic acid)

M6-M7(methylation of caffeoylquinic acid) and so on. Conclusion: Hydrogenation and methylation are the major metabolic pathways of I. cappa extract in rat feces.

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Related Institution

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