Effect of Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma on TGF-/Smads/Gli2/PTHrP Signaling Pathway in Nude Mice with Bone Metastasis of Breast Cancer

您当前的位置：

首页 >

文章列表页 >

Effect of Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma on TGF-/Smads/Gli2/PTHrP Signaling Pathway in Nude Mice with Bone Metastasis of Breast Cancer

更新时间：2024-01-04

- Effect of Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma on TGF-/Smads/Gli2/PTHrP Signaling Pathway in Nude Mice with Bone Metastasis of Breast Cancer
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 24, Pages: 128-132(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017240128
  CLC： R285.5
- Published：2017
- 稿件说明：
移动端阅览
CHENG Xu-feng, ZHANG Xin-feng, LIU Qi, et al. Effect of Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma on TGF-/Smads/Gli2/PTHrP Signaling Pathway in Nude Mice with Bone Metastasis of Breast Cancer[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(24): 128-132.
DOI：

CHENG Xu-feng, ZHANG Xin-feng, LIU Qi, et al. Effect of Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma on TGF-/Smads/Gli2/PTHrP Signaling Pathway in Nude Mice with Bone Metastasis of Breast Cancer[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(24): 128-132. DOI： 10.13422/j.cnki.syfjx.2017240128.

摘要

目的：研究附子-白术药对对乳腺癌骨转移裸鼠的保护作用及其作用机制。方法：裸鼠随机分为正常组、模型组、唑来膦酸组、附子-白术药对组，采用左心室注射乳腺癌细胞MDA-MB-231BO法建立骨转移模型，并进行相应干预。观察裸鼠骨转移程度，微计算机断层扫描（Micro-CT）检测裸鼠胫骨矿物质密度（BMD），骨体积（BV），选取感兴趣区域（ROI）体积（TV），计算骨体积分数BV/TV，抗酒石酸酸性磷酸酶染色法（TRAP）观察骨转移灶中破骨细胞表达，蛋白免疫印迹法（Western blot）法检测转化生长因子-β1（TGF-β1），Smad3，Smad4，胶质瘤相关癌基因同源蛋白2（Gli2）和甲状旁腺激素相关蛋白（PTHrP）的表达。结果：与模型组比较，附子-白术药对组骨转移程度显著降低（P<0.01），裸鼠胫骨BMD，BV/TV显著升高（P<0.01），TRAP （+）细胞数量显著减少（P<0.01），骨转移灶中TGF-β1，Smad3，Gli2和PHTrP表达均显著降低（P<0.01），Smad4表达均明显升高（P<0.05）。结论：附子-白术药对可减轻乳腺癌骨转移裸鼠溶骨性损伤，其机制可能与其上调Smad4蛋白表达，下调TGF-β1，Smad3，Gli2和PTHrP蛋白表达，调控TGF-β1/Smads/Gli2/PTHrP信号通路有关。

Abstract

Objective: To investigate the therapeutic efficacy of Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma on nude mice with bone metastasis of breast cancer and its mechanism. Method: Nude mice were randomly divided into normal group

model group

zoledronate group and Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group. Except for normal group

MDA-MB-231BO cells were injected into the left ventricle to create the model of bone metastasis of breast cancer. Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group nude mice were orally administered with Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma. Normal group

model group and zoledronate group nude mice were orally administered with physiological saline

and zoledronate was injected into the abdominal cavity in zoledronate group. The degree of bone metastases was evaluated. Bone mineral density (BMD)

bone volume (BV)

range of interests (ROI) and total volume (TV) were evaluated by micro-CT. Osteoclasts in bone metastasis were evaluated by tartrate resistant acid phosphatase dye (TRAP). Transforming growth factor-β1(TGF-β1)

drosophila mothers against decapentaplegic protein 3 (Smad3)

Smad4

Glioma-associated oncogene homologue 2 (Gli2) and Parathyroid hormone-related protein (PTHrP) in bone metastasis tissues were evaluated by Western blot. Result: The degree of bone metastases

BMD and BV/TV in Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group were higher than those of model group (P<0.01). The number of TRAP (+) cells in Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group was lower than that of model group (P<0.01). The expression of TGF-β1

Smad3

Gli2 and PHTrP protein expressions of bone metastases tissues in Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group were lower than those of model group (P<0.01)

and the expression of Smad4 in Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group was higher than that of model group (P<0.05). Conclusion: Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma has a therapeutic effect on soluble osseous injury in nude mice with bone metastasis of breast cancer

and the mechanisms of action may be that Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma could up-regulate Smad4

down-regulate TGF-β1

Smad3

Gli2 and PTHrP

and regulate TGF-β1/Smads/Gli2/PTHrP signaling pathway.

关键词

Keywords

references

0

Views

5

下载量

5

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Related Articles

Effect of Bushen Huoxuetang on Expression of Bax/Bcl-2 and Cleaved Caspase-3 in Nude Mice with Bone Metastasis of Breast Cancer

Related Author

ZHU Rong

GUO Xuebin

YANG Manying

CHEN Xuezhen

HU Yudie

YANG Zheng

YUAN Bo

HU Jinhui

Related Institution

Yiyang First Hospital of Tradition Chinese Medicine

Hunan University of Chinese Medicine

The First Hospital of Hunan University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰