ZHANG Cang-man, LU Jing-guang, XIE Qi-shi, et al. Establishment of Fingerprint and Determination of Kinsenoside of Herba Anoectochili from Different Sources by UHPLC-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(1): 31-37.
DOI:
ZHANG Cang-man, LU Jing-guang, XIE Qi-shi, et al. Establishment of Fingerprint and Determination of Kinsenoside of Herba Anoectochili from Different Sources by UHPLC-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(1): 31-37. DOI: 10.13422/j.cnki.syfjx.2018010031.
Establishment of Fingerprint and Determination of Kinsenoside of Herba Anoectochili from Different Sources by UHPLC-TOF-MS
Objective:To provide chemical evidence for the quality control of Herba Anoectochili through chemical profiling and quantitative analysis of 14 batches of herbs by UHPLC-TOF-MS technique. Method:Chemical constituents were profiled by UHPLC-TOF-MS fingerprint.Compounds in the fingerprint were assigned on the basis of accurate mass.The contents of kinsenoside was determined by using[M+HCOO]- ion.The obtained fingerprints of 14 batches of Herba Anoectochili were evaluated by similarity investigation and cluster analysis. Result:The UHPLC-TOF-MS fingerprint of Herba Anoectochili was established and thirty-six chemical constituents in the fingerprint were identified.The chemical profiling and quantitative analysis showed that the chemical components of Herba Anoectochili were significantly influenced by place of origin and cultivation time.The content range of kinsenoside was 4.85%-16.18%. Conclusion:The established fingerprint and determination of kinsenoside is feasible and stable
which is suitable for the quality control of Herba Anoectochili.
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