Objective:To investigate the constituents of Punica granatum flower. Method:The P. granatum flowers were extracted with 60% ethyl alcohol and condensed to a small amount of volume. The extraction was carried out by petroleum ether

dichloromethane

ethyl acetate and n-butyl alcohol in turn. The compounds from ethyl acetate extract were isolated and purified by silica gel

semi-preparative HPLC

Sephadex LH-20 and recrystallization methods

while their structures were identified on the basis of spectral analysis and comparison of their spectral data with those in the literature. Result:Twelve compounds were isolated and identified respectively as gallic acid (1)

ethyl gallate (2)

luteolin (3)

8-O-methylretusin (4)

7-hydroxy-4'

6-dimethoxyisoflavone (5)

formononetin (6)

tricetin (7)

taraxerone (8)

oleanolic acid (9)

ursolic acid (10)

D-dulcitol (11)

and daucosterol (12). Conclusion:The compounds 4-8 as well as 11 were isolated for the first time from P. granatum flower (ie. Uighur medicine Gulina)

and compounds 5

6 and 8 were isolated for the first time from this genus.