Metabolites and Metabolic Pathway Analysis of Brazilin in Rats by UPLC-Q-TOF-MS/MS

LIANG Guang-yan; WU Yun-shan; CHEN Wei-ying; LIU Bo; GUO De-an

doi:10.13422/j.cnki.syfjx.2018010067

您当前的位置：

首页 >

文章列表页 >

Metabolites and Metabolic Pathway Analysis of Brazilin in Rats by UPLC-Q-TOF-MS/MS

更新时间：2024-01-04

- Metabolites and Metabolic Pathway Analysis of Brazilin in Rats by UPLC-Q-TOF-MS/MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 1, Pages: 67-73(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2018010067
  CLC： R285.5
- Published：2018
- 稿件说明：
移动端阅览
LIANG Guang-yan, WU Yun-shan, CHEN Wei-ying, et al. Metabolites and Metabolic Pathway Analysis of Brazilin in Rats by UPLC-Q-TOF-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(1): 67-73.
DOI：

LIANG Guang-yan, WU Yun-shan, CHEN Wei-ying, et al. Metabolites and Metabolic Pathway Analysis of Brazilin in Rats by UPLC-Q-TOF-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(1): 67-73. DOI： 10.13422/j.cnki.syfjx.2018010067.

摘要

目的：通过研究巴西苏木素在大鼠体内的代谢产物，探析其代谢途径。方法：采用UPLC-Q-TOF-MS/MS对灌胃巴西苏木素（30 mg·kg-1）后大鼠的生物样品（粪便、尿液、血浆、胆汁）进行分析，流动相0.1%甲酸水溶液-乙腈梯度洗脱，流速0.4 mL min-1，电喷雾离子源，负离子模式，信息依赖性采集模式进行检测，离子喷雾电压-4.5 kV，离子源温度500℃，碰撞电压100 eV，碰撞能量-35 eV，锥孔气流50 L·h-1，m/z 100~1 100，使用PeakView 1.2软件进行数据处理，提取给药样品的总离子流图谱与空白生物样品对比，通过保留时间、精确相对分子质量及二级质谱的比较与分析，筛选并鉴定其可能的代谢产物。结果：共检测出23个代谢产物，这23个代谢产物是巴西苏木素在大鼠体内甲基化、脱氧、缩氢、羧酸化、水合作用、硫酸化、葡糖糖醛酸化后产生的，其中以硫酸化、葡糖糖醛酸化为主。结论：本实验首次对巴西苏木素在大鼠体内的代谢情况进行研究，能够为该药物的药效学和药理学研究提供一定的物质基础。

Abstract

Objective:The metabolites of brazilin were detected and identified in vivo. Method:The metabolic profiles of brazilin in plasma

urine

bile and feces samples after oral administration (30 mg·kg-1) were analyzed by UPLC-Q-TOF-MS/MS.The mobile phase was consisted of water(containing of 0.1% formic acid)-acetonitrile and eluted in gradient mode.The mass was detected in negative ion mode and information dependent acquisition(IDA) model was used.The data were analyzed by PeakView 1.2 software.Total ion chromatogram of sample was extracted and compared with the blank biological sample

possible metabolites were screened and identified by comparison and analysis of retention time

precise molecular mass

elemental composition and secondary mass spectrometry. Result:Twenty-three metabolites of brazilin were detected by comparing with the blank sample.The pathways to these metabolites were methylation

deoxygenation

hydrogenation

carboxylation

hydration

sulfation and glucuronide conjugation

in which sulfation and glucuronide conjugation were the main transitions. Conclusion:This study provides the metabolism of brazilin in rats for the first time

which can be useful for its pharmacology and pharmacodynamics.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Analysis on Metabolites and Metabolic Pathways of Harmine in Rats by UPLC-Q-TOF-MS

Analysis of Excretion Behavior of GK-A from Ginkgo Semen in Urine and Bile of Rats

Identification of Metabolites of Rutin in Rats by UPLC-Q-TOF/MS

Urine and Bile Excretion of Senkyunolide I in Rats

Related Author

CARTIERA Kurban

WANG Changhong

XU Nan

XU Qinwei

TENG Liang

GAO Huijing

Qing ZHANG

Qing WANG

Related Institution

State Key Laboratory of Pathogenesis， Prevention and Treatment of High Incidence Diseases in Central Asia， The First Affiliated Hospital of Xinjiang Medical University

Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine

School of Pharmacy，Xinjiang Medical University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Dongzhimen Hospital, Beijing University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰