Objective:To study the effect of spleen tonifying traditional Chinese medicine(TCM) Weichang'an granule on human gastric cancer xenografts in nude mice. Method:The nude mice models of human gastric cancer were established in nude mice and randomly divided into normal saline group (0.5 mL/mouse)

Weichang'an decoction group (35.49 g·kg-1) and Weichang'an granule group (3.54 g·kg-1). The tumor weight of nude mice between various groups was compared

and the expression levels of Ki67 and proliferating cell nuclear antigen (PCNA) in nude mice were compared by immunohistochemistry. The morphology of tumor cells was detected under transmission electron microscope

terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay was used to detect the apoptosis of tumor cells in each group

and serum tumor markers and enzymatic markers were detected by enzyme linked immunosorbent assay (ELISA). Result:The average tumor weight in the Weichang'an decoction group and the Weichang'an granule group were lower than those in normal saline group (P<0.01). The positive expression rates of PCNA and Ki67 were significantly lower than those in normal saline group (P<0.01)

transmission electron microscopy showed that the apoptosis rate and the necrosis rate of the subcutaneous tumor cells were significantly higher than those in normal saline group

ELISA showed tumor markers and CEA

CA199

CA724 enzymes

lactate dehydrogenase gastrointestinal (LDH) and alkaline phosphatase (ALP) levels of the Weichang'an decoction group and the Weichang'an granule group were lower than those of the normal saline group (P<0.05). Conclusion:Weichang'an granule and Weichang'an decoction can inhibit the growth of gastric cancer implanted in nude mice by inhibiting tumor cell proliferation and inducing apoptosis of tumor cells and promoting their differentiation

the specific mechanism shall be further studied.