JIA Ping, Chen Gang. Effect and Mechanism of Xitongwan on Gouty Arthritis Induced by Monosodium Urate in Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(1): 96-101.
DOI:
JIA Ping, Chen Gang. Effect and Mechanism of Xitongwan on Gouty Arthritis Induced by Monosodium Urate in Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(1): 96-101. DOI: 10.13422/j.cnki.syfjx.2018010096.
Effect and Mechanism of Xitongwan on Gouty Arthritis Induced by Monosodium Urate in Rats
Objective:To observe the effect and pharmacological mechanism of Xitongwan on monosodium urate (MSU)-induced gouty arthritis. Method:Rats were intragastrically treated with Xitongwan (200
400
800 mg·kg-1) for consecutively 7 days. MSU-induced gouty arthritis in rats was prepared through intra-articular injection with MSU crystals in the left ankle joint on the 5th day. Paw volume was measured at 0
6
12
24
48 h
as well as gait score was calculated at 24 h after MSU induction. Histological score in ankle joint was calculated by hematoxylin and eosin staining. Osteoclast formation in ankle joint was detected by tartrate-resistant acid phosphatase (TRAP) staining. Expressions of tumor necrosis factor (TNF)-α
interleukin (IL)-1β
IL-6 and nucleotide-binding domain and leucine-rich repeat region containing family of receptor protein 3 (NLRP3) in articular tissues were analyzed by immunohistochemistry staining or Western blot assay. Result:Intra-articular injection with MSU crystals led to apparently elevated paw volume and gait score compared with control rats. Meanwhile
histological score
osteoclast formation and expressions of TNF-α
IL-1β
IL-6 and NLRP3 of gouty arthritis rats were significantly increased compared with control rats. However
treatment with Xitongwan significantly alleviated paw volume and decreased gait score in gouty arthritis rats compared with control rats. Meanwhile
treatment with Xitongwan significantly reduced histological score
osteoclast formation and expressions of TNF-α
IL-1β
IL-6 and NLRP3 in gouty arthritis rats compared with control rats. Conclusion:Xitongwan could attenuate the inflammatory development and structural damage by inhibiting the production of inflammatory mediators in gouty arthritis rats.
Influence of Rupeng 15 Powder on COX-2, PGE Protein and mRNA Expression Levels in Rat with Mono Sodium Urate Crystals-induced Gouty Arthritis
Total Saponins of Dioscoreae Nipponicae Rhizoma Alleviates Gouty Arthritis by Down-regulating COX-2-mediated M1 Macrophage Reprogramming
Mechanism of Traditional Chinese Medicine Against Gouty Arthritis via Regulating Nrf2 Signaling Pathway: A Review
Qingre Sanzhuo Decoction Treats Gouty Arthritis Combined with Hyperuricaemia in Rats via NLRP3/ASC/Caspase-1 Pathway
Treatment of Hyperuricemia and Gouty Arthritis by Buyang Huanwu Tongfeng Decoction via Inhibition of PPAR-γ/NF-κB/AGEs/RAGE Pathway Based on Network Pharmacology
Related Author
KOU Yi-ying
LI Yong-fang
YANG Mei
LYU Hui-ling
LI Rui-lian
HUANG Lin
LIU Shumin
SUN Huijuan
Related Institution
Institute of Chinese Medicine,Heilongjiang University of Chinese Medicine
Graduate School of Heilongjiang University of Chinese Medicine
The Second People's Hospital of Fujian University of Traditional Chinese Medicine
Fujian University of Traditional Chinese Medicine
Gansu Provincial Hospital of Traditional Chinese Medicine