Effect of Tangshenqing No.1 on Expression of MCP-1 and RANTES in Renal Tissues of Diabetic Nephropathy Rats

SUN Cui-ying; WANG Jie; ZHANG Cheng-cheng; BAI Yu; FANG Tie-sheng; BAI Hua; ZHANG Xiao-xu; HE Yu-xi; WANG Da-li; ZHANG Zheng-ju; MENG Feng-xian

doi:10.13422/j.cnki.syfjx.2018010102

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Effect of Tangshenqing No.1 on Expression of MCP-1 and RANTES in Renal Tissues of Diabetic Nephropathy Rats

更新时间：2024-01-04

- Effect of Tangshenqing No.1 on Expression of MCP-1 and RANTES in Renal Tissues of Diabetic Nephropathy Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 1, Pages: 102-108(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2018010102
  CLC： R-332;R285.5
- Published：2018
- 稿件说明：
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SUN Cui-ying, WANG Jie, ZHANG Cheng-cheng, et al. Effect of Tangshenqing No.1 on Expression of MCP-1 and RANTES in Renal Tissues of Diabetic Nephropathy Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(1): 102-108.
DOI：

SUN Cui-ying, WANG Jie, ZHANG Cheng-cheng, et al. Effect of Tangshenqing No.1 on Expression of MCP-1 and RANTES in Renal Tissues of Diabetic Nephropathy Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(1): 102-108. DOI： 10.13422/j.cnki.syfjx.2018010102.

摘要

目的：观察中药糖肾清1号高、中、低剂量对糖尿病肾病肾组织局部CC族细胞趋化因子表达的影响。方法：建立糖尿病肾病大鼠模型，设正常组、模型组、卡托普利组、糖肾清1号高、中、低剂量组，正常组和模型组每日予去离子水10 mL·kg-1灌胃；卡托普利组每日予卡托普利2.33 mg·kg-1灌胃，糖肾清1号高、中、低剂量组每日分别予糖肾清1号颗粒187，93.5，46.75 mg·kg-1灌胃，连续给药12周后取材。采用时实荧光定量PCR（Real-time PCR）检测肾组织单核细胞趋化蛋白-1（MCP-1）和调节正常T细胞表达和分泌的细胞因子（RANTES）mRNA转录，蛋白免疫印迹法（Western blot）检测MCP-1蛋白表达水平，酶联免疫吸附法（ELISA）测定RANTES蛋白表达水平。结果：糖肾清1号可以下调肾组织的MCP-1，RANTES mRNA转录和蛋白表达（P<0.05，P<0.01），并且呈量效依赖性，肾组织MCP-1和RANTES的表达量为糖肾清1号高剂量组 < 中剂量组 < 低剂量组，中药各组以糖肾清1号高剂量组疗效明显。结论：糖肾清1号可通过抑制肾组织MCP-1，RANTES mRNA转录和蛋白表达，延缓糖尿病肾病的发生发展。

Abstract

Objective:To observe the effect of Tangshenqing No.1 high

medium and low doses (traditional Chinese medicine) on the expression of group CC cytokines in renal tissues of rats with diabetic nephropathy. Method:Diabetic nephropathy rat model was established

and then the rats were divided into normal control group

model control group

Captopril group

Tangshenqing No.1 high

medium and low dose treatment groups. The rats in normal control group and model control group received intragastric administration of 10 mL·kg-1 deionized water daily; captopril 2.33 mg·kg-1 was given daily in Captopril group; Tangshenqing No.1 high

medium and low dose groups respectively received 187

93.5

46.75 mg·kg-1 Tangshenqing No.1.Samples were taken after 12 weeks of successive administration. The mRNA transcription of monocyte chemoattractant protein-1(MCP-1) and regulated upon activation normal T cell expressed and secreted (RANTES) in renal tissues were detected by Real-time polymerase chain reaction (Real-time PCR). The expression level of MCP-1 protein was detected by Western blot

while the expression level of RANTES protein was determined by enzyme linked immunosorbent assay (ELISA). Result:Tangshenqing No.1 could down-regulate the mRNA transcription and protein expression levels of MCP-1 and RANTES in renal tissues (P<0.05

P<0.01) in a dose-dependent manner. The expression levels of MCP-1 and RANTES in renal tissues were as follows:Tangshenqing No.1 high dose group < middle dose group < low dose group. The curative effect was most obvious in Tangshenqing No.1 high dose group among the Chinese medicine treatment groups. Conclusion:Tangshenqing No.1 can inhibit the occurence and development of diabetic nephropathy by inhibiting the expression of MCP-1

RANTES mRNA and protein in renal tissues.

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