Effect and Mechanism of Micrococtis Folinm on Mouses with Non-alcoholic Fatty Liver Disease

SU Shi-zhen; XIANG Dong-yu; LIU Xiao-qing; JING Xue-ning; MENG Fan-wei

doi:10.13422/j.cnki.syfjx.2018010130

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Effect and Mechanism of Micrococtis Folinm on Mouses with Non-alcoholic Fatty Liver Disease

更新时间：2024-01-04

- Effect and Mechanism of Micrococtis Folinm on Mouses with Non-alcoholic Fatty Liver Disease
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 1, Pages: 130-135(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2018010130
  CLC： R285.5
- Published：2018
- 稿件说明：
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SU Shi-zhen, XIANG Dong-yu, LIU Xiao-qing, et al. Effect and Mechanism of Micrococtis Folinm on Mouses with Non-alcoholic Fatty Liver Disease[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(1): 130-135.
DOI：

SU Shi-zhen, XIANG Dong-yu, LIU Xiao-qing, et al. Effect and Mechanism of Micrococtis Folinm on Mouses with Non-alcoholic Fatty Liver Disease[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(1): 130-135. DOI： 10.13422/j.cnki.syfjx.2018010130.

摘要

目的：探讨布渣叶对非酒精性脂肪性肝病（non-alcoholic fatty liver disease，NAFLD）模型小鼠的肝脏是否具有保护作用及其机制。方法：将BALB/c小鼠随机分为正常组、模型组、辛伐他汀组（2.5 mg·kg-1），布渣叶低、高剂量组（3.5，7.0 g·kg-1），用高糖高脂饲料喂养12周制备NAFLD模型，灌胃给药，每天1次，28 d测定甘油三酯（triglyceride，TG），胆固醇（cholesterol，TC），高密度脂蛋白（high-density lipoprotein cholesterol，HDL-C），低密度脂蛋白（low-density lipoprotein cholesterol，LDL-C），丙氨酸氨基转氨酶（alanine aminotransferase，ALT），天门冬氨酸氨基转移酶（aspartate aminotransferase，AST），计算肝指数（肝湿重/体重×100%）；黄嘌呤氧化酶法测定肝匀浆中超氧化物歧化酶（superoxide dismutase，SOD）活性、硫代巴比妥酸法测定丙二醛（malondialdehyde，MDA）含量；用蛋白免疫印迹法（Western blot）检测肝组织中脂联素受体2（adiponectin receptor 2，Adipo R2）蛋白表达；光镜观察肝组织形态改变。结果：与正常组比较，模型组小鼠TC，TG，LDL-C，AST，ALT水平及肝指数显著升高（P<0.01），HDL-C含量下降（P<0.01）；肝组织中SOD活性下降（P<0.01），MDA含量显著升高（P<0.01）；肝组织中Adipo R2蛋白表达减少（P<0.01），肝脏脂肪变明显（P<0.01）。与模型组比较，布渣叶可降低血清TC，TG，LDL-C，AST，ALT水平及肝指数（P<0.01）；降低肝组织中MDA含量（P<0.01），提高SOD活性（P<0.01）；肝组织中Adipo R2蛋白表达增加（P<0.05）；肝细胞脂肪变性程度明显减轻（P<0.01）。结论：布渣叶对NAFLD模型小鼠具有降脂、保肝作用，其机制可能与抑制氧化应激、增加Adipo R2的表达有关。

Abstract

Objective:To explore the protective effect and mechanism of Micrococtis Folinm on liver of Mouse with non-alcoholic fatty liver disease (NAFLD). Method:BALB/c mouse were randomly divided into normal group

model group

simvastatin group (2.5 mg·kg-1) and high and low-dose Micrococtis Folinm groups (3.5

7.0 g·kg-1). The NAFLD model was prepared through intragastric administration with high-sugar and high-fat diets for 12 weeks

once a day. Serum triglyceride (TG)

cholesterol (TC)

high-density lipoprotein cholesterol (HDL-C)

low density lipoprotein cholesterol (LDL-C)

alanine amino transaminase (ALT) and aspartate aminotransferase (AST) were detected

and liver index were calculated (liver wet weight/body weight×100%) at the 28th day. Activity of superoxide dismutase (SOD) and malondialdehyde (MDA) in liver homogenate were determined by xanthine oxidase and thiobarbital acid method

respectively. Adiponectin receptor 2 (Adipo R2) in liver tissues was detected by western blotting. The morphological changes in liver tissues were observed under light microscope. Result:Compared with the normal group

the model group showed higher levels of serum TC

LDL-C

AST and ALT (P<0.01)

and a lower level of serum HDL-C (P<0.01)

higher liver index (P<0.01)

lower SOD activity (P<0.01) and higher MDA (P<0.01) in liver homogenate

decreased Adipo R2 protein expression in liver tissues (P<0.05)

and severer hepatic steatosis (P<0.01). Compared with the model group

the Micrococtis Folinm group showed lower levels of serum TC

LDL-C

AST and ALT (P<0.01)

lower liver index (P<0.01)

lower MDA (P<0.01) and increased SOD activity (P<0.01) in liver homogenate

increased Adipo R2 protein expression in liver tissues (P<0.05)

and significantly relieved liver steatosis (P<0.01). Conclusion:Micrococtis Folinm has lipid-lowering and hepatoprotective effects on NAFLD mice

and its mechanism may be related to the inhibition of oxidative stress and the increase of Adipo R2 expression.

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