LIU Jin-xin, LI Geng, CHEN Cai-xia, et al. Identification of DNA Barcoding of Atractylodis Rhizoma Seedlings by ITS2 Sequence[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(2): 34-38.
DOI:
LIU Jin-xin, LI Geng, CHEN Cai-xia, et al. Identification of DNA Barcoding of Atractylodis Rhizoma Seedlings by ITS2 Sequence[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(2): 34-38. DOI: 10.13422/j.cnki.syfjx.2018020034.
Identification of DNA Barcoding of Atractylodis Rhizoma Seedlings by ITS2 Sequence
Objective:In order to guarantee the species correction of Atractylodis Rhizoma seedlings
a molecular identification method with internal transcribed spacer 2(ITS2) as DNA barcode has been tested. Method:Here
28 original plant specimens of Atractylodis Rhizoma and their common adulterants have been collected to construct its standard reference ITS2 sequence database.Fifty-two Atractylodis Rhizoma seedlings have been collected from Hebei province
Shandong province
Shanxi province
Inner Mongolia autonomous region
Liaoning province and Hubei province to verify their original species.Their ITS2 sequences have been obtained after DNA extraction
polymerase chain reaction(PCR) and bi-directional sequencing.The intra-species and inter-species genetic distances were calculated by PUAP 4.0 software.The neighbor-joining(NJ) trees were constructed by MEGA 7.0 software. Result:The stable sequence divergence has been found between ITS2 barcode sequences of Atractylodis Rhizoma and their common adulterants
their NJ trees showed monophyly.With the help of standard reference ITS2 sequence database
we found 42 Atractylodis Rhizoma seedlings were from Atractylodes koreana
7 Atractylodis Rhizoma seedlings were from A. lancea
3 Atractylodis Rhizoma seedlings were from A. macrocephala. Conclusion:Atractylodis Rhizoma seedlings are mainly come from A. koreana.This result is consistent with market survey of medicinal resources
suggesting that Atractylodis Rhizoma has safety risk.The DNA barcoding method based on the ITS2 sequence can accurately distinguish between Atractylodis Rhizoma seedlings and their adulterants seedlings.
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