Objective:To establish the UPLC fingerprints of Gardeniae Fructus from different origins

and provide a more comprehensive and rapid evaluation method for the quality control of Gardeniae Fructus. Method:The batches of Gardeniae Fructus from different origins and 1 batch of Gardenia jasminoides var. grandiflora were analyzed by UPLC fingerprints by using an ACQUITY UPLC HSS T3 column (2.1 mm×100 mm

1.8 μm)

with acetonitrile-0.05% phosphoric acid as the mobile phase for gradient elution. The flow rate was 0.4 mL·min-1 and the detection wavelength was set at 265 nm. The similarity analysis

cluster analysis and principal component analysis were used to evaluate the fingerprints of the 27 batches of samples. Result:The common fingerprints for the 26 batches of Gardeniae Fructus samples were established

among which 23 peaks were common fingerprint peaks

and 8 main compounds were identified. The similarity between the fingerprint of 26 batches of Gardeniae Fructus and the control fingerprint was more than 0.96

and the similarity between the control fingerprint and the fingerprint of G. jasminoides var.grandiflora was 0.935. Gardeniae Fructus and G. jasminoides var. grandiflora between the existing samples can also be separated by cluster analysis. Principal component analysis showed a higher level of Gardeniae Fructus from Jiangxi

and the quality was basically the same. Conclusion:The UPLC fingerprint can provide a scientific evaluation for the quality control of Gardeniae Fructus.