Protective Effect of Astragaloside Ⅳ Combined with Hydroxysafflor Yellow A on Mice with Myocardial Infarction

SU Ning; LU Fen-ping; ZHU Ming-ming; ZHENG Zong-mei; SHEN Yu-jun; LIU Ting-yu; SHI Hong-fei

doi:10.13422/j.cnki.syfjx.2018020098

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Protective Effect of Astragaloside Ⅳ Combined with Hydroxysafflor Yellow A on Mice with Myocardial Infarction

更新时间：2024-01-04

- Protective Effect of Astragaloside Ⅳ Combined with Hydroxysafflor Yellow A on Mice with Myocardial Infarction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 2, Pages: 98-103(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2018020098
  CLC： R285.5
- Published：2018
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SU Ning, LU Fen-ping, ZHU Ming-ming, et al. Protective Effect of Astragaloside Ⅳ Combined with Hydroxysafflor Yellow A on Mice with Myocardial Infarction[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(2): 98-103.
DOI：

SU Ning, LU Fen-ping, ZHU Ming-ming, et al. Protective Effect of Astragaloside Ⅳ Combined with Hydroxysafflor Yellow A on Mice with Myocardial Infarction[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(2): 98-103. DOI： 10.13422/j.cnki.syfjx.2018020098.

摘要

目的：观察黄芪甲苷联合羟基红花黄色素A预处理对心肌梗死小鼠心梗面积、心肌细胞凋亡及炎症因子表达的影响。方法：50只（SPF级）C57小鼠雌雄各半，随机分为假手术组（Sham），心肌梗死模型组（MI），黄芪甲苷预处理组（MI+AsⅣ，3.5 mg·kg-1），羟基红花黄色素A预处理组（MI+HSYA，10 mg·kg-1），黄芪甲苷+羟基红花黄色素A联合预处理组（MI+AsⅣ+HSYA，3.5 mg·kg-1+10 mg·kg-1）。造模前连续腹腔注射7 d，每天1次；结扎左冠状动脉前降支建立小鼠心肌梗死模型，用伊文思蓝-TTC双染色方法区分心肌梗死后心肌的梗死区、缺血危险区（AAR）和左心室总面积（LV）；ImageJ软件计算心肌梗死面积，蛋白免疫印迹法（Western blot）检测心肌细胞中B细胞淋巴癌/白血病-2基因（Bcl-2），Bcl-2相关X蛋白（Bax），活化的天冬氨酸蛋白水解酶-3（cleaved-Caspase-3）的表达水平，酶联免疫吸附法（ELASA）检测血清白细胞介素-6（interleukin-6，IL-6），肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）的含量。结果：与模型组相比，黄芪甲苷预处理组、羟基红花黄色素A预处理组及联合预处理组均可显著减小小鼠心肌梗死面积，且联合预处理组效果优于单药预处理组（P<0.05，P<0.01）；与假手术组相比，模型组的缺血面积和梗死面积增大，单独给药组与联合给药组的缺血面积及梗死面积均减小（P<0.05，P<0.01）；与模型组与假手术相比，黄芪甲苷联合羟基红花黄色素A可上调心肌细胞中抗凋亡蛋白Bcl-2的表达，下调促凋亡蛋白cleaved-Caspase-3和Bax的表达；黄芪甲苷与羟基红花黄色素A联合可降低心肌梗死小鼠血清中IL-6，TNF-α的含量（P<0.05，P<0.01）。结论：黄芪甲苷联合羟基红花黄色素A可减小小鼠心肌梗死面积，抑制心肌梗死后的心肌细胞凋亡及炎症反应，发挥保护心肌损伤的作用。

Abstract

Objective:To observe the effect of pretreatment with astragaloside Ⅳ combined with hydroxy safflower yellow A on myocardial infarction area

cardiomyocyte apoptosis and expression of inflammatory cytokines in mice with myocardial infarction. Method:Totally 50 SPF grade C57 mice (half male and half female) were randomly divided into sham operation group (Sham)

myocardial infarction model group (MI)

astragaloside pretreatment group (MI + As Ⅳ

3.5 mg·kg-1)

hydroxy safflower yellow A pretreatment group (MI + HSYA

10 mg·kg-1)

and astragaloside Ⅳ + hydroxy safflower yellow A pretreatment group (MI + As Ⅳ + HSYA

3.5 mg·kg-1+10 mg·kg-1). Intraperitoneal injection lasted for 7 days before modeling

once a day. The model of myocardial infarction was established by ligating left anterior descending coronary artery. The infarction area

ischemic area and total left ventricle area of myocardium were distinguished by evans blue-TTC double staining method. ImageJ software was used to calculate the myocardial infarction area

the expressions of Bax

Bcl-2 and cleaved-Caspase-3 in myocardium were detected by Western blot. The levels of serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA) content. Result:Compared with the model group

the pretreatment group and the combined pretreatment group could significantly reduce the myocardial infarction area of the mice

and the effect of the combined treatment group was better than that of the single pretreatment group. Compared with the sham group

the ischemic area and infarction area of the model group were increased

and the ischemic area and infarction area of the combination group and the combined administration group were decreased(P<0.05

P<0.01). Compared with the model group and the sham operation group

astragaloside Ⅳ combined with hydroxy safflower yellow A up-regulates the expression of anti-apoptotic protein Bcl-2 incardiomyocytes

and down-regulates the expressions of cleaved-Caspase-3 and Bax(P<0.05

P<0.01). Astragaloside Ⅳ combined with hydroxysafflor yellow A can reduce the levels of IL-6 and TNF-α in serum of mice with myocardial infarction(P<0.05

P<0.01). Conclusion:Astragaloside Ⅳ combined with hydroxy safflower yellow A can reduce the myocardial infarction area in mice

and inhibit myocardial cell apoptosis and inflammatory response after myocardial infarction

with an effect in protecting myocardial injury.

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