Effect of Tongxie Yaofang with or Without Saposhnikoviae Radix on Colonic Visceral Hypersensitivity and Brain-gut Peptide in Irritable Bowel Syndrome Rats
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Effect of Tongxie Yaofang with or Without Saposhnikoviae Radix on Colonic Visceral Hypersensitivity and Brain-gut Peptide in Irritable Bowel Syndrome Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 24, Issue 2, Pages: 152-157(2018)
LIANG Rui-feng, GE Wen-jing, WANG Hui-sen, et al. Effect of Tongxie Yaofang with or Without Saposhnikoviae Radix on Colonic Visceral Hypersensitivity and Brain-gut Peptide in Irritable Bowel Syndrome Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(2): 152-157.
DOI:
LIANG Rui-feng, GE Wen-jing, WANG Hui-sen, et al. Effect of Tongxie Yaofang with or Without Saposhnikoviae Radix on Colonic Visceral Hypersensitivity and Brain-gut Peptide in Irritable Bowel Syndrome Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(2): 152-157. DOI: 10.13422/j.cnki.syfjx.2018020152.
Effect of Tongxie Yaofang with or Without Saposhnikoviae Radix on Colonic Visceral Hypersensitivity and Brain-gut Peptide in Irritable Bowel Syndrome Rats
Objective:To investigate the effect of Tongxie Yaofang with or without Saposhnikoviae Radix on colonic visceral hypersensitivity and brain-gut peptide in the diarrhea-predominant irritable bowel syndrome (D-IBS) rats. Method:Newly born SD rats were randomly divided into control group
model group
Tongxie Yaofang without Saposhnikoviae Radix group
Tongxie Yaofang group
Tongxie Yaofang with double Saposhnikoviae Radix group. Except for the control group
the rats in the other groups were separated from their mothers and given acetic acid by gavage to establish the D-IBS model. These rats in the each group were administered with corresponding drugs for two weeks. The score of abdominal withdrawal reflex (AWR) and the fecal moisture content in rats were measured. The contents of 5-hydroxytryptamine (5-HT) and P substance (SP) were determined by enzyme linked immunosorbent assay (ELISA). The expressions of corticotropin releasing factor (CRH) were determined by immunohistochemical technique. Result:The fecal moisture content
minimum volume threshold
5-HT and SP levels and expression of CRH in experimental groups were significantly lower than those in model group (P<0.05
P<0.01). Compared with the Tongxie Yaofang without Saposhnikoviae Radix group
the fecal moisture content
5-HT and SP levels and expression of CRH in the Tongxie Yaofang group and the Tongxie Yaofang with double Saposhnikoviae Radix group decreased significantly
while the AWR score increased in different degrees (P<0.05
P<0.01). Conclusion:Saposhnikoviae Radix can enhance the therapeutic effect of Tongxie Yaofang decoction in inhibitng visceral hypersensitivity and regulating brain-gut peptide at different targets in D-IBS rats.
Effect of Tongxie Yaofang with or Without Saposhnikoviae Radix on Water Metabolism and 5-HT Pathway in Irritable Bowel Syndrome Rats
Effect of Tongxie Yaofang on p38 MAPK Pathway Associated Protein and Its Target Gene Expression in Colon Tissues of D-IBS Rats with Liver-stagnation and Spleen-deficiency
Clinical Observation on the Therapeutic Effect of Tongxie Yaofang Combined with Microecological Preparation for Irritable Bowel Syndrome
Multi-target Network Construction and Mechanism Analysis of Main Effective Components of Tongxie Yaofang
Related Author
ZHANG Wei
GE Wen-jing
WANG Hui-sen
ZHANG Xue-xia
LIU Ming
CUI Wei-feng
WANG Jun
LI Geng-sheng
Related Institution
Institute of Chinese Materia Medica, Henan Academy of Traditional Chinese Medicine
Gansu University of Chinese Medicine
Medicine College of Hexi University
School of Traditional Chinese Medicine,Capital Medical University