Analysis of Spectrum-effect Relationship of Antioxidant Activity of Sanguotang Based on Partial Least Square Method

JIANG Hong; SHI Ya-jun; ZHAO Sheng-yu; LIN Jun-zhi; HAN Li; ZHANG Ding-kun; YANG Ming; LI Hong-yan

doi:10.13422/j.cnki.syfjx.2018030008

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Analysis of Spectrum-effect Relationship of Antioxidant Activity of Sanguotang Based on Partial Least Square Method

更新时间：2024-01-04

- Analysis of Spectrum-effect Relationship of Antioxidant Activity of Sanguotang Based on Partial Least Square Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 3, Pages: 8-12(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2018030008
  CLC： R284.1
- Published：2018
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JIANG Hong, SHI Ya-jun, ZHAO Sheng-yu, et al. Analysis of Spectrum-effect Relationship of Antioxidant Activity of Sanguotang Based on Partial Least Square Method[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(3): 8-12.
DOI：

JIANG Hong, SHI Ya-jun, ZHAO Sheng-yu, et al. Analysis of Spectrum-effect Relationship of Antioxidant Activity of Sanguotang Based on Partial Least Square Method[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(3): 8-12. DOI： 10.13422/j.cnki.syfjx.2018030008.

摘要

目的：通过建立三果汤HPLC指纹图谱并测定其抗氧化活性，探讨其抗氧化活性的物质基础，为建立三果汤的质控评价指标提供依据。方法：采用正交试验，通过回流提取得到9个三果汤样品，采用HPLC进行分析得到指纹图谱，1，1-二苯基-2-三硝基苯肼（DPPH）法测定其抗氧化活性。应用偏最小二乘法研究三果汤中特征色谱峰和抗氧化活性的相关性，筛选活性色谱峰。结果：建立了三果汤HPLC指纹图谱方法，相似度均>0.9，确定了其中27个色谱峰为共有峰，其中12个峰的变量投影重要性（VIP）均>1.0，说明三果汤指纹图谱中12个色谱峰对三果汤抗氧化活性发挥重要活性。通过与对照品比对，确定了峰5为没食子酸、峰10为没食子儿茶素、峰20为柯里拉京、峰23为表儿茶素没食子酸酯、峰27为鞣花酸。结论：三果汤抗氧化作用是多个成分协同起效的结果，通过谱-效关系分析得到三果汤的抗氧化物质基础，可为三果汤的质量控制提供实验依据。

Abstract

Objective:To study on the material basis of antioxidant activity of Sanguotang by establishing its HPLC fingerprints and determining its antioxidant activity. Method:Nine samples of Sanguotang were extracted by reflux method and orthogonal test.Fingerprints were analyzed by HPLC

1-diphenyl-2-trinitrophenylhydrazine(DPPH) assays was established to determine the antioxidant activity of Sanguotang.The spectrum-effect correlation was investigated by partial least square method

and the active chromatographic peaks were screened out. Result:HPLC fingerprints of Sanguotang was established

and the similarities were more than 0.90.And 27 common peaks were identified

the variable projection importance(VIP) values of these 12 peaks were more than 1.0

indicating that the 12 peaks played an important role in the antioxidant activity of Sanguotang.By comparison with the reference substances

the peak 5

27 were determined as gallic acid

gallocatechin

corilagin

epicatechin gallate and ellagic acid

respectively. Conclusion:The antioxidant activity of Sanguotang is the result of synergistic effect of multiple components

its material basis can be analyzed by spectrum-effect relationship

which can provide basis for the quality control of Sanguotang.

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