Simultaneous Determination of Four Components in  by HPLC with Wavelength Switching

JIANG Jian-ping; WANG Mei-qi; MA Wen-fang; CHEN Qiu-ling; WEI Ming-mei; CHEN Hui

doi:10.13422/j.cnki.syfjx.2018030072

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Simultaneous Determination of Four Components in by HPLC with Wavelength Switching

更新时间：2024-01-04

- Simultaneous Determination of Four Components in by HPLC with Wavelength Switching
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 3, Pages: 72-76(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2018030072
  CLC： R284.1
- Published：2018
- 稿件说明：
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JIANG Jian-ping, WANG Mei-qi, MA Wen-fang, et al. Simultaneous Determination of Four Components in by HPLC with Wavelength Switching[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(3): 72-76.
DOI：

JIANG Jian-ping, WANG Mei-qi, MA Wen-fang, et al. Simultaneous Determination of Four Components in by HPLC with Wavelength Switching[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(3): 72-76. DOI： 10.13422/j.cnki.syfjx.2018030072.

摘要

目的：建立滇桂艾纳香药材中原儿茶酸、绿原酸、咖啡酸和芦丁4种成分的含量测定方法，同步测定4种成分含量，为滇桂艾纳香药材的质量控制提供参考。方法：采用HPLC转换波长法，Inertsil ODS-3色谱柱（4.6 mm×250 mm，5 μm），以乙腈（A）-0.1%磷酸水溶液（B）为流动相进行梯度洗脱（0~25 min，10%~18% A；25~45 min，18%~40% A），检测波长256 nm （0~15 min，原儿茶酸），327 nm （15~30 min，绿原酸、咖啡酸），360 nm （30~60 min，芦丁），柱温20℃，流速1.0 mL·min-1。结果：滇桂艾纳香中原儿茶酸、绿原酸、咖啡酸、芦丁进样量分别在0.007~0.13 μg （r=0.999 98），0.219~4.38 μg （r=0.999 94），0.014~0.28 μg （r=0.999 97），0.049~0.98 μg （r=0.999 97）与峰面积呈良好的线性关系，平均加样回收率分别为98.84%（RSD 2.2%），101.16%（RSD 0.9%），98.00%（RSD 2.8%），101.73%（RSD 1.4%）。含量测定结果显示，百色平果县所产药材中原儿茶酸、绿原酸和芦丁含量均较高，百色靖西县和四川药材咖啡酸含量较高。结论：所建立的方法稳定可靠，灵敏度高，重复性好，可同时测定滇桂艾纳香药材中原儿茶酸、绿原酸、咖啡酸和芦丁4种成分的含量，可为滇桂艾纳香药材质量评价提供参考。

Abstract

Objective:To determinate the contents of protocatechuic acid

chlorogenic acid

caffeic acid and rutinin in Blumea riparia by HPLC

and provide references for its quality control. Method:The HPLC analysis was carried out by Inertsil ODS-3 column (4.6 mm×250 mm

5 μm) with acetonitrile (A)-0.1% phosphoric acid solution (B) as the mobile phase for gradient elution. The detection wavelength was set at 256 nm (0-15 min

protocatechuic acid)

327 nm (15-30 min

chlorogenic acid and caffeic acid)

and 360 nm (30-60 min

rutin). In addition

the flow rate was 1.0 mL·min-1 and column temperature was 20℃. Result:Protocatechuic acid

chlorogenic acid

caffeic acid and rutin showed good linearity in the range of 0.007-0.13 μg (r=0.999 98)

0.219-4.38 μg (r=0.999 94)

0.014-0.28 μg (r=0.999 97) and 0.049-0.98 μg (r=0.999 97) respectively

and the recovery of those 4 components were 98.84% (RSD 2.2%)

101.16% (RSD 0.9%)

98.00% (RSD 2.8%) and 101.73% (RSD 1.4%) respectively. The content determination results showed that the contents of protocatechuic acid

chlorogenic acid and rutinin were higher in Pingguo samples; and the content of caffeic acid was higher in Jingxi and Sichuan samples. Conclusion:The established method can be used to determine the contents of protocatechuic acid

chlorogenic acid

caffeic acid and rutin. Such stable

reliable and repeatable analytical model can provide a reference for the quality evaluation and its control on Blumea riparia.

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