Objective:To investigate the effect of Shuwei decoction on expressions of IP3 receptor (inositol 1

4

5-triphosphate receptor

IP3R)

ryanodine receptor(RyR)protein and mRNA expression of intestinal cells of Cajal (ICC) of rats model of functional dyspepsia (FD)

in order to explore the mechanism of Shuwei decoction in the treatment of FD in the aspect of calcium channel adjustment. Method:Totally 15 Wistar rats were randomly divided into the blank group

the model group and the Shuwei decoction group (30.68 g·kg-1). The model group and the Shuwei decoction group were treated by the compound etiological modeling method for 21 day to replicate the FD model. After intragastric administration for 14 d

blood was collected to prepare drug-containing serum. Intestinal smooth muscle in subcultured ICC cells was taken from 2-3-week-old Wistar rats

and were divided into blank serum group (group A)

model serum group (group B)

Shuwei decoction 5%-containing serum group (group C)

Shuwei decoction 10%-containing serum group (group D)

and Shuwei decoction 15%-containing serum group (group E). When the cells were fused to 60%-80%

corresponding serums were added to each group and incubated for 24 h. IP3R and RyR were detected by Western blot

and IP3R and RyR mRNA expressions were detected by Real-time PCR. Result:Western blot results showed that compared with group A

the expressions of IP3R-1

IP3R-2

IP3R-3

RyR-1

RyR-2

RyR-3B protein in group B were significantly decreased (P<0.01). Compared with group B

the expressions of IP3R

RyR protein in group D and E were significantly increased (P<0.05

P<0.01). Real-time PCR results showed that mRNA expressions of IP3R and RyR were significantly decreased compared with group A (P<0.01). Compared with group B

mRNA expressions of IP3R and RyR in group D and E were significantly increased (P<0.01). Conclusion:Shuwei decoction has a therapeutic effect on functional dyspepsia by up-regulating IP3R

RyR and mRNA expression.