Objective:To study the effect of Jianpi Xiaoai recipe in vivo on apoptosis of subcutaneous tumor cells and expression of related proteins in mice with colorectal carcinoma

and investigate its mechanisms of anti metastasis and recurrence of colorectal cancer. Method:By subcutaneous injection of CT-26 colon cancer cells to construct models of subcutaneous colorectal cancer

and then these mice were randomly divided into five groups:model group

Jianpi Xiaoai low(15.75 g·kg-1)

medium(31.5 g·kg-1)

high (63 g·kg-1) dose groups

and 5-Fu group. After treatment for 14 days

the animals were sacrificed

and their tumors were taken and weighed. Then the tumors were sheared to detect the apoptosis of every group by flow cytometry. In addition

expression of related proteins was detected by Western blot. Result:As compared with model group

tumor weights of Jianpi Xiaoai recipe high

middle and low dose groups were reduced (P<0.05)

and the rates of apoptosis were increased (P<0.05); the expression levels of X proteins related to B cell lymphoma-2 (Bax)

Caspase-3

Caspase-8 were increased (P<0.05)

while the expression of B cell lymphoma-2 (Bcl-2) was decreased (P<0.05). In addition

the increase in Bax

Caspase-3

and Caspase-8 in Jianpi Xiaoai recipe high dose group was more obvious than that in the low and middle dose groups. Conclusion:Jianpi Xiaoai recipe can inhibit the growth of subcutaneous tumor cells in mice with colorectal cancer by promoting its apoptosis

and its mechanism may be related to regulating the expressions of apoptosis related proteins.