LYU Shu-quan, ZHANG Shu-fang, WANG Zhen-qiang, et al. Protective Effect of Jianpi Gushen Huayu Decoction on Diabetic Nephropathy Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(3): 142-147.
DOI:
LYU Shu-quan, ZHANG Shu-fang, WANG Zhen-qiang, et al. Protective Effect of Jianpi Gushen Huayu Decoction on Diabetic Nephropathy Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(3): 142-147. DOI: 10.13422/j.cnki.syfjx.2018030142.
Protective Effect of Jianpi Gushen Huayu Decoction on Diabetic Nephropathy Rats
Objective:To study the effect of Jianpi Gushen Huayu decoction (JGHD) in alleviating and protecitng renal tissue rats with diabetic nephropathy. Method:Totally 60 male SD rats were randomly divided into 6 groups:blank group
model group
ramipril group
low-dose JGHD group
middle-dose JGHD group
and high-dose JGHD group. After modeling
triglyceride
total cholesterol
creatinine
urea and 24-hour urine microalbumin were observed in 6 groups of rats. The morphological changes in kidney were observed under light microscope. Immunohistochemical staining was used to observe transforming growth factor-β1 (TGF-β1)
metallothionein-1 (MT-1)
α-smooth muscle actin (α-SMA)
light chain-Ⅱ(LC3-Ⅱ)
and autophagy-related protein Beclin. TGF-β1 mRNA expression was detected by reverse transcription PCR (RT-PCR) in rats. Result:Jianpi Gushen Huayu decoction could reduce the levels of serum triglyceride
total cholesterol
creatinine
urea
24 h urinary albumin
and effectively inhibit levels of TGF-β1
MT-1
α-SMA
LC3-Ⅱ
Beclin and TGF-β1 mRNA expression. It could improve the pathological changes in renal tissues. Conclusion:Jianpi Gushen Huayu decoction could reduce serum triglyceride
total cholesterol
creatinine
urea
24 h urinary microalbumin
TGF-β1
MT-1
α-SMA
LC3-Ⅱ
Beclin and TGF-β1 mRNA expression
with the effect in alleviating and protecting the pathological changes in renal tissues.
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Related Institution
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