Anti-inflammatory and Anti-oxidative Stress Damage of Catechin Gallate on Diabetic Myocardial in C57 Mice

REN Zhong-kun; ZHANG Zhi-bi; LIU Yan-hong; ZHANG Rong-ping; YANG Hui

doi:10.13422/j.cnki.syfjx.2018030148

您当前的位置：

首页 >

文章列表页 >

Anti-inflammatory and Anti-oxidative Stress Damage of Catechin Gallate on Diabetic Myocardial in C57 Mice

更新时间：2024-01-04

- Anti-inflammatory and Anti-oxidative Stress Damage of Catechin Gallate on Diabetic Myocardial in C57 Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 3, Pages: 148-153(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2018030148
  CLC： R285.5
- Published：2018
- 稿件说明：
移动端阅览
REN Zhong-kun, ZHANG Zhi-bi, LIU Yan-hong, et al. Anti-inflammatory and Anti-oxidative Stress Damage of Catechin Gallate on Diabetic Myocardial in C57 Mice[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(3): 148-153.
DOI：

REN Zhong-kun, ZHANG Zhi-bi, LIU Yan-hong, et al. Anti-inflammatory and Anti-oxidative Stress Damage of Catechin Gallate on Diabetic Myocardial in C57 Mice[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(3): 148-153. DOI： 10.13422/j.cnki.syfjx.2018030148.

摘要

目的：探讨儿茶素没食子酸酯（EGCG）对糖尿病小鼠高糖诱导的心肌组织氧化应激损伤的影响。方法：采用雄性C57小鼠给予高糖高脂饮食6周后，按70 mg·kg-1的标准腹腔注射链脲佐菌素（STZ），连续5 d。空腹血糖（FBG）>16.7 mmol·L-1视为造模成功。后随机分为空白组，模型组，EGCG低、中、高剂量组（50，100，200 mg·kg-1），N-乙酰半胱氨酸（NAC，100 mg·kg-1）组，腹腔注射，隔天1次，连续4周。分别测定小鼠体重、心脏质量、心脏质量指数、血糖值；镜下观察心肌细胞及组织病理变化；酶联免疫吸附法（ELISA）检测心肌组织活性氧（ROS），超氧化物歧化酶（SOD），丙二醛（MDA）及炎性因子白细胞介素（IL）-1β，IL-6，肿瘤坏死因子（TNF）-α的含量；蛋白免疫印迹法（Western blot）测定心肌组织中NF-E2相关因子2（Nrf2）介导的抗氧化通路中血红素氧合酶-1（HO-1）和醌氧化还原酶1（NQO1）的表达。结果：与模型组相比，EGCG （100 mg·kg-1）组能一定程度降低血清中的FBG水平（P<0.05）；抑制心肌细胞肥大及心肌组织纤维化程度，增强SOD活性，降低MDA，ROS含量（P<0.05）；降低组织中炎性因子IL-1β，IL-6，TNF-α的含量（P<0.05）；激活心肌细胞核内Nrf2介导的抗氧化通路，作用优于强抗氧化剂NAC。结论：EGCG对高糖诱导的心肌细胞损伤具有保护作用，其机制可能与EGCG的抗氧化应激、抗炎作用有关。

Abstract

Objective:To explore the protective effect of catechin gallate (EGCG) on diabetic myocardial injury in C57 mice. Method:The diabetic cardiomyopathy injury model in C57 mice was induced by injected with streptozocin (STZ

70 mg·kg-1) and provided with high-diet food. The DCM mice were randomly divided into 6 groups

namely blank group

model group

EGCG group (50

100

200 mg·kg-1)

and N-acetyl-L-cysteine(NAC) group (100 mg·kg-1). Each group was put to death on the 14th day after intraperitoneal injection. The level of fasting blood-glucose (FBG) was detected by kit; reactive oxygen (ROS)

malondialdehyde (MDA)

superoxide dismutase (SOD)

interniukin (IL)-1β

IL-6 and tumor necrosis factor (TNF)-α were measured by enzyme linked immunosorbent assay (ELISA) kit. Myocardial pathological changes of cardiomyopathy injury were observed under microscope; Nrf2 and HO-1

NQO1 protein kinases were detected by Western blot. Result:Compared with the model group

EGCG (100 mg·kg-1) can decrease the level of FBG in DCM C57 mice and inhibit the activities of IL-1β

IL-6

MDA

ROS in cardiac muscle tissues (P<0.05); increase the activity of SOD in cardiac muscle tissues (P<0.05); increase the expression of protein kinase of Nrf2

HO-1

NQO1 in cardiac muscle tissues (P<0.05). Moreover

EGCG (100 mg·kg-1) can restrain the cardiomyocyte hypertrophy and interstitial fibrosis apparently in cardiac muscular tissues. The findings showed that EGCG was better than NAC. Conclusion:EGCG has a protective effect on diabetic cardiomyopathy in C57 mice. The mechanism may be correlated with its anti-inflammatory and anti-oxidative stress damage action.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Role of Oxidative Stress in Ulcerative Colitis and Traditional Chinese Medicine Prevention and Treatment： A Review

Effect of Nitrogen Application on Antioxidant Enzyme System of Acanthopanax senticosus

Effect of Extract on Blood Pressure and Lipid Peroxidation of Target-organ in Renal Hypertension Rats

Effect of Dahuang Zhechong Wan on Formation Stage of Pulmonary Fibrosis in Rats

Related Author

CHEN Bo

SUN Xitong

DONG Xinqian

WANG Wei

SONG Hongquan

ZHAO Jiting

ZHENG Ya

ZHANG Honghao

Related Institution

Affiliated Hospital of Shandong University of Traditional Chinese Medicine

Shandong University of Traditional Chinese Medicine

School of Forestry, Northeast Forestry University

The First Affiliate Hospital of Clinical Medicine of Guangdong Pharmaceutical University

School of Pharmacy, Heilongjiang University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰