Objective:To investigate the detoxication effect and efficacy of water extract from Veratrilla baillonii(WVBF) with compatibility of total alkaloids from Aconitum brachypodum(CFA). Method:The cardiovascular toxicity was evaluated by using zebra fish cardiovascular model; up-and-down method was used to calculate the median lethal dose (LD50) of CFA and WVBF of different compatibility proportions; then acetic acid induced writhing response and hot plate method were used to observe and compare the central and peripheral analgesic effects of CFA-WVBF 1:2 group and CFA-WVBF 1:5 group. Result:In cardiovascular toxicity evaluation experiment

if CFA was used alone

it had no effect on heart rate and did not induce arrhythmia of zebra fish except 200 mg·L-1 group; 66.6

200 mg·L-1 groups induced toxicity phenotype (P<0.05). In different compatibilities experiment

all the experimental groups induced obvious cardiovascular toxicity except CFA-WVBF 1:5 group (P<0.05). The LD50 of CFA-WVBF compatibilities (5:1

2:1

1:2

1:5) was increasing in turn

and all of them were higher than the LD50 of CFA alone (P<0.05). In the central analgesia experiment

CFA-WVBF 1:2 group and CFA-WVBF 1:5 group can enhance foot pain threshold

and the analgesia effects of CFA-WVBF 1:2 group (CFA-WVBF

20 mg·kg-1) were better than those of CFA (20 mg·kg-1) alone group and aspirin group (200 mg·kg-1)

but the analgesia percentage in CFA-WVBF 1:2 group was similar to that in CFA group and higher than that in aspirin group (P<0.05) after 90 minutes

indicating that CFA-WVBF 1:2 group could reduce toxicity and keep efficacy. The analgesia percentage in CFA-WVBF 1:5 group was higher than that in the CFA alone (20 mg·kg-1) group

and was gradually increased after 30

60

90 minutes

indicating that CFA-WVBF 1:5 group could reduce toxicity

enhance efficacy and extend efficacy (P<0.05). In peripheral analgesia experiment

different proportions of CFA and WVBF groups could inhibit acetic acid induced writhing response. The writhing inhibition rate in CFA-WVBF 1:2 group was lower than that of CFA alone group (P<0.05); and the writhing inhibition rate in CFA-WVBF 1:5 group was slightly higher than that of CFA alone group (P<0.05)

indicating that the CFA-WVBF 1:2 group could reduce toxicity and decrease efficacy but the CFA-WVBF 1:5 group could reduce toxicity and enhance analgesic efficacy slightly (P<0.05). Conclusion:Different compatibilities of CFA and WVBF can decrease CFA-induced cardiovascular toxicity in zebra fish and acute toxicity in mice; CFA-WVBF 1:2 group and CFA-WVBF 1:5 group can increase central analgesic efficacy

and CFA-WVBF 1:5 group also can enhance peripheral analgesic efficacy. Therefore

CFA-WVBF 1:5 group shows the best effect in decreasing toxicity and increasing efficacy.