Objective: To screen the specific binding components in Buyang Huanwu Tang(BYHWT) on neuron-like PC12 cells

and to provide experimental basis for further study and application of this compound. Method: A method on analysis neuron-like PC12 cells specificity binding components in BYHWT by solid-phase extraction(SPE)

neuron-like PC12 cells binding assay combined with SPE was employed

and the samples were analyzed by UPLC-MS/MS

mobile phase was methanol-0.1% formic acid aqueous solution under gradient elution

the injection volume was 3 μL and the flow rate was set as 0.3 mL·min-1.The mass spectrometric conditions were set as following:atomizer pressure and auxiliary gas pressure of 379.2 kPa

air curtain gas pressure of 241.3 kPa

ion source temperature at 500 ℃

spray voltage of －4.5 kV. Result: The neuron-like PC12 cells specificity binding components in BYHWT were found as follows:6-hydroxykaempferol-3

6

7-tri-O-glucoside

6-hydroxykaempferol-3

6-di-O-glucoside and calycosin-7-O-β-D-glucoside. Conclusion: These three components may be the important compositions for neuroprotective function in BYHWT.