Objective: To explore a extracting method of genomic DNA from Ranae Oviductus and lie a foundation for further molecular experiments of this medicinal herb. Method: Sodium dodecyl sulfate(SDS) method

improved SDS method

Chelex-100 method

guanidine thiocyanate(GuSCN) method

kit method and improved kit method were used to extract genomic DNA from Ranae Oviductus.The yield and quality of genomic DNA were evaluated by agarose gel electrophoresis

ultraviolet spectrophotometry and polymerase chain reaction(PCR) amplification of primer with cytochrome C oxidase Ⅰ(COⅠ) sequence. Result: Chelex-100 method

improved SDS method

GuSCN method and improved kit method could extract genomic DNA from Ranae Oviductus

while its yields of SDS method and kit method were little.The quality and yield of genomic DNA extracted by improved SDS method were high with complex operation.The improved kit method cost too much and the yield of DNA was low.The Chelex-100 method was easy to operate and cost less time

but the quality of DNA was poor.DNA extracted by GuSCN method contained many PCR inhibitors. Conclusion: The genomic DNA extacted by Chelex-100 method

improved SDS method and improved kit method all can meet the demands of PCR amplification

suitable method can be selected to extract genomic DNA from Ranae Oviductus according to the actual conditions of the laboratory.