Effect and Mechanism of Total Flavonoids from Smilacis Glabrae Rhizoma in Treatment of Gouty Arthritis Based on NLRP3 Inflammasomes Axis

JIN Xiao-min; ZHANG Xiao-xi; GUO Lu; LI Yang-lei; XING Meng-yu; TIAN Chong-mei; XIA Dao-zong

doi:10.13422/j.cnki.syfjx.2018040090

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Effect and Mechanism of Total Flavonoids from Smilacis Glabrae Rhizoma in Treatment of Gouty Arthritis Based on NLRP3 Inflammasomes Axis

更新时间：2024-01-04

- Effect and Mechanism of Total Flavonoids from Smilacis Glabrae Rhizoma in Treatment of Gouty Arthritis Based on NLRP3 Inflammasomes Axis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 4, Pages: 90-95(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2018040090
  CLC： R285.5
- Published：2018
- 稿件说明：
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JIN Xiao-min, ZHANG Xiao-xi, GUO Lu, et al. Effect and Mechanism of Total Flavonoids from Smilacis Glabrae Rhizoma in Treatment of Gouty Arthritis Based on NLRP3 Inflammasomes Axis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(4): 90-95.
DOI：

JIN Xiao-min, ZHANG Xiao-xi, GUO Lu, et al. Effect and Mechanism of Total Flavonoids from Smilacis Glabrae Rhizoma in Treatment of Gouty Arthritis Based on NLRP3 Inflammasomes Axis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(4): 90-95. DOI： 10.13422/j.cnki.syfjx.2018040090.

摘要

目的：研究土茯苓总黄酮（TFSG）对急性痛风性关节炎小鼠的抗炎作用，并初步探讨其作用机制。方法：将90只雄性C57BL/6小鼠随机分为正常组，模型组，秋水仙碱组（0.65 mg·kg-1），土茯苓总黄酮低、中、高剂量组（100，300，500 mg·kg-1），每组15只。除正常组外，其余各组采用尿酸钠结晶复制小鼠痛风性关节炎模型，测定小鼠踝关节肿胀度；酶联免疫吸附法（ELISA）检测不同组别滑膜组织中白细胞介素-1β（IL-1β），IL-6，肿瘤坏死因子-α（TNF-α）水平，蛋白免疫印迹法（Western blot）测定滑膜组织中核苷酸结合寡聚化结构域样受体蛋白3（NLRP3），凋亡相关斑点样蛋白（ASC），半胱氨酸天冬氨酸蛋白酶-1（Caspase-1）的蛋白表达，实时荧光定量PCR（Real-time PCR）检测NLRP3，ASC，Caspase-1 mRNA水平的表达。结果：与正常组比较，模型组小鼠踝关节肿胀度明显增加（P<0.01），模型组小鼠滑膜组织中IL-1β，IL-6，TNF-α水平显著升高（P<0.01），NLRP3，ASC，Caspase-1蛋白表达和mRNA水平显著升高（P<0.01）；与模型组比较，土茯苓总黄酮组能够显著降低小鼠踝关节肿胀度（P<0.01），滑膜组织中IL-1β，IL-6，TNF-α水平以及NLRP3，ASC，Caspase-1蛋白水平和mRNA水平显著降低（P<0.01）。结论：土茯苓总黄酮具有一定的抗痛风性关节炎作用，其作用机制与NLRP3炎性体轴有关。

Abstract

Objective: To observe the anti-inflammatory effect of total flavonoids from Smilacis Glabrae Rhizoma (TFSG) on acute gouty arthritis and its mechanism. Method: Totally 90 C57BL/6 mice were randomly divided into the normal group

the model group

the colchicine group (0.65 mg·kg-1)

and the TFSG group (100

300

500 mg·kg-1)

with 15 mice in each group. The acute gouty arthritis model was induced with monosodium urate (MSU) crystals

except for the normal group

and the mice's ankle swelling degree was measured. The mice's synovium tissues were collected to detect the inflammatory mediators interleukin-1β (IL-1β)

IL-6 and tumor necrosis factor-α (TNF-α) by enzyme-linked immunosorbent assay. The protein expressions of Nod-like receptor protein 3 (NLRP3)

apoptosis-associated speck-like protein (ASC)

and cysteine aspartic acid protease-1 (Caspase-1) were detected by Western blot

and the mRNA expressions of NLRP3

ASC

and Caspase-1 messenger in synovium tissues were detected by Real-time PCR. Result: Compared with the normal group

mice ankle swelling degree of the model group increased significantly (P<0.01)

and the levels of IL-1β

IL-6

TNF-α and the protein and mRNA expressions of NLRP3

ASC and Caspase-1 were significantly increased (P<0.01). Compared with the model group

the TFSG group could significantly reduce ankle swelling degree and IL-1β

IL-6 and TNF-α levels (P<0.01)

as well as NLRP3

ASC

Caspase-1 protein and mRNA expressions(P<0.01). Conclusion: TFSG has an anti-gouty arthritis effect

and its mechanism is correlated with NLRP3 inflammasome axis.

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