Objective: To observe the effect of Erzhiwan on uterus coefficient

estrodiol (E2) in serum

pathological changes in tissues of uterus and vagina

and expressions of estrogen receptors (ERα and ERβ) of immature mice

in order to investigate the estrogen-like mechanism

and provide experimental evidence for the clinical application of Erzhiwan in the treatment of perimenopausal syndrome. Method: Forty female Kunming mice were randomly divided into 4 groups: normal group

estrogen receptor agonist group (EV

0.154 mg·kg-1) and Erzhiwan (1.365

2.73 g·kg-1) groups. Seven days later

the serum level of E2 was detected by enzyme-linked immunosorbent assay (ELISA). The pathological changes in immature mouse tissues were observed by hematoxylin-eosin(HE) staining. The expressions of ERα and ERβ were detected by Western blot. Result: Compared with normal group

Erzhiwan groups showed a significant increase in the uterus coefficient (P<0.05) and a decrease in the level of E2 (P<0.05)

and the growth and development of uterus and vagina

and increase of the expressions of ERα and ERβ in vagina of immature mice

with a similar effect with EV. Conclusion: Erzhiwan plays an estrogen-like effect by up-regulating the expression of estradiol in blood and the expressions of estrogen receptors in target organ.