Effect of Polydatin on MCF-7 Cells Proliferation and Its Underlying Mechanism

QIN Qing-hong; HUANG Min; TAN Qi-xing; LIAN Bin; WEI Chang-yuan

doi:10.13422/j.cnki.syfjx.2018040143

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Effect of Polydatin on MCF-7 Cells Proliferation and Its Underlying Mechanism

更新时间：2024-01-04

- Effect of Polydatin on MCF-7 Cells Proliferation and Its Underlying Mechanism
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 4, Pages: 143-148(2018)
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.2018040143
  CLC： R737.9
- Published：2018
- 稿件说明：
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QIN Qing-hong, HUANG Min, TAN Qi-xing, et al. Effect of Polydatin on MCF-7 Cells Proliferation and Its Underlying Mechanism[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(4): 143-148.
DOI：

QIN Qing-hong, HUANG Min, TAN Qi-xing, et al. Effect of Polydatin on MCF-7 Cells Proliferation and Its Underlying Mechanism[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(4): 143-148. DOI： 10.13422/j.cnki.syfjx.2018040143.

摘要

目的：研究白藜芦醇苷（polydatin）在体外对乳腺癌细胞MCF-7增殖的作用，进一步探索其潜在的机制。方法：设定空白组及白藜芦醇苷（0.2，0.4，1.2，2.0，2.8 mg·L-1）组，5-氟尿嘧啶组，采用噻唑蓝（MTT）比色法测定不同质量浓度白藜芦醇苷对MCF-7细胞的生长抑制率；应用细胞克隆集落形成实验验证白藜芦醇苷对MCF-7细胞增殖的抑制作用；通过实时荧光定量PCR（Real-time PCR）检测5-氟尿嘧啶（1.0 μg·L-1）和白藜芦醇苷（0.2，0.4，1.2 mg·L-1）对DNA聚合酶δ催化亚基P125（P125），P125编码基因（POLD1），p53基因（p53），细胞周期依赖性蛋白激酶抑制因子p21 mRNA表达水平的影响；应用蛋白质免疫印迹法（Western blot）检测白藜芦醇苷（0.2，0.4，1.2 mg·L-1）对P125，p53，p21蛋白表达水平的影响。结果：与空白组比较，白藜芦醇苷（0.2，0.4，1.2，2.0，2.8 mg·L-1）在体外对MCF-7细胞具有明显的抑制作用（P<0.05，P<0.01），并且这种抑制作用呈浓度依赖性。与空白组比较，白藜芦醇苷（0.2，0.4，1.2 mg·L-1）均能明显抑制细胞集落的形成（P<0.05，P<0.01）。与空白组比较，5-氟尿嘧啶组和白藜芦醇苷（0.4，1.2 mg·L-1）能够显著抑制POLD1 mRNA表达水平，同时显著抑制P125 mRNA和蛋白表达水平，明显升高p53，p21 mRNA和蛋白表达水平（P<0.05，P<0.01）。与5-氟尿嘧啶组比较，白藜芦醇苷（1.2 mg·L-1）对POLD1，P125，p53，p21 mRNA和蛋白表达水平影响均无显著差异。结论：白藜芦醇苷在体外能够显著抑制MCF-7细胞的增殖，其潜在机制可能与提高p53表达水平，抑制POLD1表达，促进p21表达有关。

Abstract

Objective: To study the effect of polydatin on the proliferation of MCF-7 cells

in order to further explore its underlying mechanism. Method: MCF-7 cells were treated with polydatin at different doses (0

0.2

0.4

1.2

2.0

2.8 mg·L-1). The inhibitory rate of the cells was detected by methylthiazolydiphenyl-tetrazolium bromide (MTT) assay after treatment for 48 h. To further verify the inhibitory effect of polydatinon on the proliferation of MCF-7 cells

the cell colonies were detected by the colony formation assay after treatment with polydatin (0.2

0.4

1.2 mg·L-1) for 10 days. After treatment with polydatin for 48 h

the effect of polydatin (0.2

0.4

1.2 mg·L-1) on mRNA expressions of DNA polymerase δ catalytic subunit gene POLD1

P125

p53 and p21 were detected by Real-time PCR. Protein expressions of P125

p53 and p21 were detected by Western blot. Result: Polydatin at different doses (0.2

0.4

1.2

2.0

2.8 mg·L-1) showed an inhibitory effect on the proliferation of MCF-7 cells in a concentration-dependent manner (P<0.05

P<0.01). After administration for 10 days

polydatin (0.2

0.4

1.2 mg·L-1) significantly inhibited the formation of cell colonies

compared with control group (P<0.05

P<0.01). After treatment for 48 h

mRNA expressions of POLD1 and P125 were significantly lower in polydatin (0.4

1.2 mg·L-1) groups and 5-FU group than control group

while mRNA expressions of p53 and p21 were increased significantly (P<0.05

P<0.01). After treatment for 48 h

protein expression of P125 was obviously lower in polydatin (0.4

1.2 mg·L-1) groups and 5-FU group than control group

while protein expressions of p53 and p21 were increased significantly. The effect of polydatin on mRNA and protein expressions of PLOD1

P125

p53 and p21 in polydatin (1.2 mg·L-1) group had no difference with 5-FU group. Conclusion: Polydatin shows a significantly inhibitory effect on the proliferation of MCF-7 cells. Its underlying mechanisms may be associated with the up-regulation of p53

the inhibition of expression of POLD1 and the promotion of p21.

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