Objective: To analyze the inhibitory effects of Yiqi Huoxue Jiedu decoction on proliferation of ovarian epithelial carcinoma ID-8 cells in vitro

observe the effect of this method on angiogenesis of human umbilical vein endothelial cells (HUVECs)

and provide experimental basis for its efficacy. Method: Cell counting kit-8 (CCK-8) was used to testify the inhibitory effect of Yiqi Huoxue Jiedu decoction on the proliferation of ovarian cancer ID-8 cells; decoction serum

regulatory T cells (Tregs) isolated by immune magnetic MACS MicroBeads

as well as vascular endothelial growth factors (VEGF) were co-cultured

and then the angiogenesis of HUVEC was observed. Result: As compared with blank control group (fetal bovine serum group)

drug serum groups with concentration of 10% and 20% showed inhibitory effects on ID-8 cells

and this effect was most significant at 96 h. Serum group with concentration of 20% showed remarkable advantages against 10% group at 72 h (P<0.01) and 96 h (P<0.05). HUVECs cultivated with Treg cells exhibited a better angiogenesis process. On the other hand

the vascular network was not obvious in drug serum group

and the inhibitory effect was most significant in anti-VEGF+Yiqi Huoxue Jiedu decoction. Conclusion: Yiqi Huoxue Jiedu decoction can restrict the multiplication of ovarian epithelial carcinoma ID-8 cells

and it could intervene the angiogenesis of HUVECs

which is probably associated with the intervention on Tregs.