LIU Hong-yu, XU Yu-qin, OUYANG Ting, et al. Identification of Saponins in Yiqi Fumai Freeze-dried Powder for Injection by HPLC-Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 7-12.
DOI:
LIU Hong-yu, XU Yu-qin, OUYANG Ting, et al. Identification of Saponins in Yiqi Fumai Freeze-dried Powder for Injection by HPLC-Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 7-12. DOI: 10.13422/j.cnki.syfjx.2018050007.
Identification of Saponins in Yiqi Fumai Freeze-dried Powder for Injection by HPLC-Q-TOF-MS
Objective:To analyze the saponins in Yiqi Fumai freeze-dried powder for injection by HPLC-Q-TOF-MS
and provide reference for quantitative study of saponins in the preparation and its pharmacodynamical material basis. Method:The fragmentation patterns of ginsenoside Rg1
Re and Rb1 were analyzed in detail
and the saponins in Yiqi Fumai freeze-dried powder for injection were identified by HPLC-Q-TOF-MS. Result:There were two main pathways for mass spectrometric cleavage of ginsenoside Rg1
Re and Rb1:①loss of all aglycones until forming a stable deprotonated parent nucleus
②aglycones fragmentation and the formation of characteristic fragment ions.On the basis of the fragmentation law of mass spectrometry
21 saponins in Yiqi Fumai freeze-dried powder for injection were identified
in addition to ginsenoside Rg1
Rb1
Re
Rc
Rd
Rf
Rg3 and notoginsenoside R1
the other 13 saponins were first reported in this preparation.At the same time
deoxyschizandrin was initially identified by literature comparison. Conclusion:Most of the saponins with high content in Yiqi Fumai freeze-dried powder for injection are identified by HPLC-Q-TOF-MS
which can provide material foundation for further investigation of this preparation.