Objective:To investigate the influence of temperature

light and pH on stability of cinobufagin

resibufogenin and total bufotenine in Bufonis Venenum extract. Method:The contents of cinobufagin and resibufogenin were determined by HPLC

the detection wavelength was 296 nm and the mobile phase was acetonitrile-water(58:42).The content of total bufotenine was determined by UV

the detection wavelength was set at 559 nm.The effect of high temperature (60

40℃)

strong light irradiation[(4 500±500) Lx] and different pH (40℃

pH of 4.32

4.99

5.73

6.47

6.97

7.75) on stability of cinobufagin

resibufogenin and total bufotenine in Bufonis Venenum extract placed 0

5

10 d was investigated. Result:The degradation of the three indicators was greater than 5% under 60℃ for 10 d.The degradations of cinobufagin and resibufogenin were both <5% under the conditions of 40℃ and (4 500±500) Lx

the degradation of total bufotenine was >5% under the above conditions.Degradation of cinobufagin at pH 5.73 was the slowest

degradation of resibufogenin was the slowest at about pH 6.47

and degradation of total bufotenine at pH 6.47 was the slowest. Conclusion:High temperature and strong light irradiation have small effect on cinobufagin and resibufogenin in Bufonis Venenum extract

but total bufotenine is affected by high temperature and strong light irradiation.The pH is the main factor affecting the stability of three index components in Bufonis Venenum extract

but different pH conditions have different effects on the stability of these three index components.