Objective:To establish a new type method for Separation-determination of stachyose in Leonuri Herba and its preparation by ion chromatography

which provides a new idea for the study of stachyose. Method:The CS12A cation exchange column (4 mm×250 mm)

CSRS 500 (4 mm) self circulation electric control mode to suppress the background conductance signal of eluent

and the suppression current is 16 mA

6 mmol·L-1 mesylate solution as eluent of isocratic eluent by isocratic elution

the flow rate is 0.8 mL·min-1

the column temperature is 30℃

and the detection method is conductivity detection. Result:According to the above chromatographic conditions

the effective separation was achieved and the rapid detection was achieved in Leonuri Herba of stachydrine.The mass concentration of stachyose is 0.41-10.23 mg

the scope of insider relations is good (r=0.999 9)

the detection limit is 0.11 mg·L

the limit of quantitation was 0.41 mg·L

reproducible test results for 7.88-8.38 mg·g-1

RSD was 2.3% (n=6)

the average recovery rate of sample recovery test results was 98.74%-101.82%

RSD was 1.2%(n=6)

the average content of stachyose in the 8 batches of leonurus japonicus from different habitats was 7.16-15.63 g·L-1

the content of in Guizhou province Xiuwen county up to 15.63 mg·g-1

and the content of Pingyi Liuyu for a minimum of 7.16 mg·g-1. Conclusion:The method has good reproducibility

high accuracy

strong specificity and simple operation in this paper.It can be used for quantitative and qualitative analysis of the stachydrine in Leonuri Herba and its preparations

and also provides a basis for the experimental study on the determination of quaternary ammonium compounds by ion chromatography.