Effect of Serum Containing Shenqi Yiliu Prescription on Proliferation and Cycle of Gastric Cancer BGC-823 Cells

PI Lin-jun; MA Chun-lin; LI Hai-long; ZENG Qing-tao; LIU Jia-nan; WU Hong-yan

doi:10.13422/j.cnki.syfjx.2018050121

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Effect of Serum Containing Shenqi Yiliu Prescription on Proliferation and Cycle of Gastric Cancer BGC-823 Cells

更新时间：2024-01-04

- Effect of Serum Containing Shenqi Yiliu Prescription on Proliferation and Cycle of Gastric Cancer BGC-823 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 5, Pages: 121-125(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2018050121
  CLC： R285
- Published：2018
- 稿件说明：
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PI Lin-jun, MA Chun-lin, LI Hai-long, et al. Effect of Serum Containing Shenqi Yiliu Prescription on Proliferation and Cycle of Gastric Cancer BGC-823 Cells[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 121-125.
DOI：

PI Lin-jun, MA Chun-lin, LI Hai-long, et al. Effect of Serum Containing Shenqi Yiliu Prescription on Proliferation and Cycle of Gastric Cancer BGC-823 Cells[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 121-125. DOI： 10.13422/j.cnki.syfjx.2018050121.

摘要

目的：探讨参芪抑瘤方含药血清对胃癌细胞BGC-823增殖和周期的影响，并探讨其作用机制。方法：以参芪抑瘤方3%，5%，10%含药血清干预BGC-823细胞后，采用3-（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴盐法检测其对细胞增殖的影响；采用流式细胞仪检测细胞周期变化；采用实时荧光定量PCR（Real-time PCR）检测细胞周期蛋白依赖性激酶（cyclin-dependent kinase inhibitor，CDKN）1B，CDKN1C mRNA表达；蛋白免疫印迹法（Western blot）检测p27，p57蛋白表达情况。结果：不同浓度含药血清作用于BGC-823细胞24，48，72 h后，细胞存活率显著降低（P<0.01），且与时间和浓度呈依赖关系；不同浓度含药血清作用于BGC-823细胞24 h后，G0/G1期细胞增多，S期细胞减少；Real-time PCR结果表明，与溶剂组和空白血清组比较，参芪抑瘤方各组CDKN1B，CDKN1C mRNA表达上调（P<0.05，P<0.01）；Western blot结果显示，与溶剂组比较，参芪抑瘤方各组p27，p57蛋白表达上调（P<0.05，P<0.01）。结论：参芪抑瘤方含药血清可以抑制胃癌BGC-823细胞的增殖，其机制可能通过调节CDKN1B，CDKN1C mRNA及p27，p57蛋白表达，进而干预细胞周期有关。

Abstract

Objective:To investigate the effect of serum containing Shenqi Yiliu prescription on inhibiting cell proliferation and blocking cell cycle of gastric cancer cell lines BGC-823

and explore its possible mechanism. Method:After being treated with 3%

5% and 10% serum containing Shenqi Yiliu prescription

gastric cancer cell lines BGC-823 were tested by 3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide for proliferation; flow cytometry was used to detect the cell cycle; Real-time PCR technique was used to detect the mRNA expression of cyclin-dependent kinase inhibitor 1B (CDKN1B) and cyclin-dependent kinase inhibitor 1C (CDKN1C) genes; and Western blot was employed to test the protein expression levels of p27 and p57. Result:After 24

72 hours treatment with different concentrations of serum containing Shenqi Yiliu prescription

the cell viability was decreased significantly (P<0.01) in a time and dose dependent manner. Flow cytometry analysis showed

when treated by different concentrations of serum containing Shenqi Yiliu prescription for 24h

BGC-823 cells count in S phase was decreased significantly and increased in G0/G1 phase. Real-time PCR results showed

as compared with blank control group and solvent group

CDKN1B and CDKN1C mRNA expression levels were increased in each Shenqi Yiliu prescription group (P<0.05

P<0.01). Western blot results showed that as compared with blank control group

p27 and p57 protein expression levels were increased significantly in each Shenqi Yiliu prescription group (P<0.05

P<0.01). Conclusion:The serum containing Shenqi Yiliu prescription can inhibit the BGC-823 cell proliferation and the mechanism may be associated with regulating CDKN1B

CDKN1C mRNA and protein expression to intervene in the cell cycle.

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