Objective:To Study the effect of Guizhou zedoary oil on death factor receptor (FAS) protein expression

Toll like receptor 2 (TLR2)

Toll like receptor 4 (TLR4) and interleukin-10 (IL-10)

C-Raf

transforming growth factor beta 1 (TGF-β1) in rectal cancer cells SW1463

and investigate the immune effect of Guizhou zedoary oil on tumor cells. Method:Guizhou zedoary oil extracted by steam distillation was divided into 80

120

160

200 mg·L-1 groups

and another blank group was also set up. After 24 h treatment on rectal cancer cell SW1463

enzyme linked immunosorbent assay (ELISA) was used to detect the different doses of Guizhou zedoary oil on the expression of IL-10; and Western blot method was used to detect Fas

TLR2

TLR4

C-Raf and TGF-β1 protein expression levels and investigate its inhibitory effect on rectal cancer cells SW1463 proliferation. Result:ELISA results showed that as compared with blank control group

IL-10 expression was down-regulated with the increase of concentration in Guizhou zedoary oil groups (F=23.11

P<0.01). Western blot results showed that the protein expression levels of TLR2

TLR4 and C-Raf were significantly decreased with the increase of drug concentration (P<0.01)

and the protein expression levels of Fas and TGF-β1 were also decreased (P<0.05). Conclusion:The immune mechanism of Guizhou zedoary oil in inhibiting SW1463 cells may be associated with down-regulation of Fas/FasL pathway

down-regulation of Toll like receptor TLR2 and TLR4 protein expression

leading to the down-reulation of relate factor TGF-β1

so that cell apoptosis is achieved to enhance the immune function.