Analysis of Material Basis and Mechanism of Ephedrae Herba-Armeniacae Semen Amarum Medicine Pair Based on Intestinal Absorption Fluid Containing Drug

XU Zhao; XIAO Xiong; HUANG Gang; LI Ying; SU Dan; LI Wen-hong

doi:10.13422/j.cnki.syfjx.20180601

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Analysis of Material Basis and Mechanism of Ephedrae Herba-Armeniacae Semen Amarum Medicine Pair Based on Intestinal Absorption Fluid Containing Drug

更新时间：2024-01-04

- Analysis of Material Basis and Mechanism of Ephedrae Herba-Armeniacae Semen Amarum Medicine Pair Based on Intestinal Absorption Fluid Containing Drug
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 6, Pages: 1-6(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.20180601
  CLC： R284.1
- Published：2018
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XU Zhao, XIAO Xiong, HUANG Gang, et al. Analysis of Material Basis and Mechanism of Ephedrae Herba-Armeniacae Semen Amarum Medicine Pair Based on Intestinal Absorption Fluid Containing Drug[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 1-6.
DOI：

XU Zhao, XIAO Xiong, HUANG Gang, et al. Analysis of Material Basis and Mechanism of Ephedrae Herba-Armeniacae Semen Amarum Medicine Pair Based on Intestinal Absorption Fluid Containing Drug[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 1-6. DOI： 10.13422/j.cnki.syfjx.20180601.

摘要

目的：探讨不同配比的麻黄-杏仁药对含药肠吸收液中化学成分含量-效应指标之间的关联性，初步阐明该药对的物质基础及作用机制。方法：选用U6（64）均匀设计表安排药对配比，采用UPLC-Q-TOF-MS/MS分析麻黄-杏仁药对含药肠吸收液中主要差异性成分及其含量；观察含药肠吸收液对大鼠气管环舒张率及组织中白细胞介素-2（IL-2），IL-4，肿瘤坏死因子-α（TNF-α）含量的影响；经多元线性回归分析效应指标与主要成分含量的相关性。结果：通过变量重要性投影值>1.5找出该药对的显著性差异成分为去甲基麻黄碱（X1），伪麻黄碱（X2），甲基麻黄碱（X3），苦杏仁苷（X4）；该药对能明显增加气管环的舒张率（Y1），升高组织中IL-2（Y2）含量，降低IL-4（Y3）与TNF-α（Y4）含量；以差异性成分含量为自变量，效应指标为因变量，得回归方程分别为Y1=-4 810.119X1+3 516.816X2-2 663.871X3-81.310X4+33.562（r=0.937），Y2=4 203.354X1-2 567.853X2+1 445.790X3+47.460X4+237.192（r=0.996），Y3=587.549X1-456.975X2+366.365X3+6.239X4+27.519（r=0.951），Y4=-21 958.981X1-35 885.568X2+40 943.441X3+944.167X4+4 465.528（r=0.968）。结论：麻黄-杏仁药对平喘的主要物质基础为去甲基麻黄碱、伪麻黄碱、甲基麻黄碱和苦杏仁苷。其平喘机制与各效应成分双向调节气道舒张与收缩功能，以及改变IL-4，IL-2，TNF-α含量等有关。

Abstract

Objective: To explore the correlation between the content of chemical components and effect index in the intestinal absorption fluid of Ephedrae Herba-Armeniacae Semen Amarum with different proportions

and clarify the material basis and mechanism of the medicine pair.Method: The U6(64) uniform design was used to arrange the proportions of this medicine pair

the major differential components in intestinal absorption fluid of this medicine pair and their contents were analyzed by UPLC-Q-TOF-MS/MS.Effect of intestinal absorption fluid containing drug on diastolic rate of tracheal ring in rats and the content of interleukin-2 (IL-2)

IL-4

tumor necrosis factor-α (TNF-α) in the rat tissue was investigated.The correlation between the effect index and the main component content was analyzed by multiple linear regression.Result: After being analyzed by the least squares regression

the significant difference components found by variable importance in the projection (VIP)>1.5 were norephedrine (X1)

pseudoephedrine (X2)

methyl ephedrine (X3) and amygdalin (X4).The medicine pair can obviously relieve the bronchial ring (Y1)

increase content of IL-2 (Y2)

decrease contents of IL-4 (Y3) and TNF-α (Y4) in the tissue.Taking contents of major differential components as independent variables (X)

effect index as dependent variables (Y)

regression equations were obtained as follows:Y1=-4 810.119X1+3 516.816X2-2 663.871X3-81.310X4+33.562(r=0.937)

Y2=4 203.354X1-2 567.853X2+1 445.790X3+47.460X4+237.192(r=0.996)

Y3=587.549X1-456.975X2+366.365X3+6.239X4+27.519(r=0.951)

Y4=-21 958.981X1-35 885.568X2+40 943.441X3+944.167X4+4 465.528(r=0.968).Conclusion: Main material bases of Ephedrae Herba-Armeniacae Semen Amarum on asthma are norephedrine

pseudoephedrine

methyl ephedrine and amygdalin

its antiasthmatic mechanism is related to bidirectional regulating airway function of each effect component

changes in IL-4

IL-2 and TNF-α content and so on.

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