Simultaneous Determination of Seven Components in Dipsaci Radix by HPLC

MA Hui-wen; Yi Hong; LI Chun; ZHANG Dong; TIAN Lian-chao; LI Yun; LIU Xiao-qian; WANG Zhi-min

doi:10.13422/j.cnki.syfjx.20180607

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Simultaneous Determination of Seven Components in Dipsaci Radix by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Seven Components in Dipsaci Radix by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 6, Pages: 55-60(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20180607
  CLC： O657.72;R284.1
- Published：2018
- 稿件说明：
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MA Hui-wen, Yi Hong, LI Chun, et al. Simultaneous Determination of Seven Components in Dipsaci Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 55-60.
DOI：

MA Hui-wen, Yi Hong, LI Chun, et al. Simultaneous Determination of Seven Components in Dipsaci Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 55-60. DOI： 10.13422/j.cnki.syfjx.20180607.

摘要

目的：建立HPLC同时测定续断中7种成分（当药苷，马钱苷，马钱苷酸，绿原酸，川续断皂苷Ⅵ，续断苷A和续断苷B）含量的方法，为全面评价和控制续断的质量提供科学依据。方法：采用HPLC测定，色谱条件为Venusil HILIC色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈（A）-0.1%磷酸水溶液（B）梯度洗脱（0~20 min，95% A；20~40 min，95%~87% A；40~64 min，87%~84% A；64~65 min，84%~78% A；65~80 min，78%~77% A），流速1.0 mL ·min-1，柱温30℃，进样量10 μL，检测波长235 nm和212 nm。结果： 7种成分的分离度良好且在各自浓度范围内与峰面积呈良好线性关系（r ≥ 0.999 9），平均回收率在99.11%~102.24%，RSD均<2.0%。13批续断样品中川续断皂苷Ⅵ的含量均符合2015年版《中国药典》规定，但各样本中7种待测成分含量差异较大。绿原酸含量与川续断皂苷Ⅵ含量呈明显正相关，而总环烯醚萜与绿原酸、川续断皂苷Ⅵ无明显相关性。结论：所建立的方法简便、准确、重复性良好，可用于续断药材的多成分同步测定，为该药材的质量控制提供参考。

Abstract

Objective: To develop a HPLC method for simultaneous determination of seven components (chiratin

loganin

loganic acid

chlorogenic acid

asperosaponin Ⅵ

dipsanoside A and dipsanoside B) in Dipsaci Radix

so as to provide scientific basis for the comprehensive quality evaluation and control of Dipsaci Radix.Method: Samples were analyzed on a Venusil HILIC column(4.6 mm×250 mm

5 μm)

and eluted with the mobile phase of acetonitrile(A) and 0.1% phosphoric acid(B) with a linear gradient mode(0-20 min

95% A;20-40 min

95%-87% A;40-64 min

87%-84% A;64-65 min

84%-78% A;65-80 min

78%-77% A)

the flow rate of the mobile phase was 1.0 mL ·min-1 with the column temperature of 30℃

and the detection wavelengths were set at 235 nm and 212 nm

respectively.Result: These seven components were well separated and showed good linearity(r ≥ 0.999 9) within the concentration ranges tested

the mean recoveries were 99.11%-102.24% with the RSD between 0.2%-1.5%.The content of asperosaponin Ⅵ in 13 samples conformed to standard in the 2015 edition of Chinese Pharmacopoeia

but the content of the seven components in each sample showed a great differences.Conclusion: The method is simple

accurate and repeatable

it can be used for the determination of multiple components in Dipsaci Radix.

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