Objective: Tagetes patula is compositae plants of the genus of Asteraceae. The modern researches showed that its flowers contained a large amount of lutein with antioxidant activity. The thiophene derivatives in the roots exhibited antimicrobial activity. The citric acid and malic acid obtained from the methanol extract of root showed hypotensive activity. The aim of this study was to investigate the chemical constituents of stems and leaves of T. patula for the first time

and to reveal the material basis for the treatment of diseases. Method: The herbs of T. patula were extracted with 95% ethanol. Then the extract was combined and evaporated in vacuum to residue

which was suspended in water and successively partitioned with ethyl acetate. Part of the ethyl acetate extract was isolated and purified by various column chromatographs such as silica gel

ODS column and preparative HPLC. Then the structures of isolated and purified compounds were determined by physical-chemical property and spectral analysis such as 13C-NMR and 1H-NMR. Result: Six compounds were identified as syringaresinol-4'-O-β-D-monoglucoside (1 )

2-methoxy-4-(2-propenyl) phenyl-β-D-glucoside (2 )

dibutyl phthalate (3 )

patuletin (4 )

β-daucostero (6 )

β-glutamyl sterol (6 )

4-allyl-2

6-dimethoxy phenyl glucoside (7 )

and 1-β-D-glucopyranosyl-2

6-dimethoxy-4-propenylphenol (8 ). Conclusion: Compounds 1

2

3

7 and 8 were isolated from the genus of Tagetes for the first time

providing certain chemical basis and foundation for the comprehensive development and the search for anti-inflammatory and antioxidant active substances in natural plants.