Protective Effect and Mechanism of Taohong Siwutang on OGD-induced Injury of Human Brain Microvascular Endothelial Cells

JI Zhao-jie; HAN Lan; WU Huan-ru; GUO Dong-dong; PENG Dai-yin

doi:10.13422/j.cnki.syfjx.20180627

您当前的位置：

首页 >

文章列表页 >

Protective Effect and Mechanism of Taohong Siwutang on OGD-induced Injury of Human Brain Microvascular Endothelial Cells

更新时间：2024-01-04

- Protective Effect and Mechanism of Taohong Siwutang on OGD-induced Injury of Human Brain Microvascular Endothelial Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 7, Pages: 95-100(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20180627
  CLC： R285
- Published：2018
- 稿件说明：
移动端阅览
JI Zhao-jie, HAN Lan, WU Huan-ru, et al. Protective Effect and Mechanism of Taohong Siwutang on OGD-induced Injury of Human Brain Microvascular Endothelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 95-100.
DOI：

JI Zhao-jie, HAN Lan, WU Huan-ru, et al. Protective Effect and Mechanism of Taohong Siwutang on OGD-induced Injury of Human Brain Microvascular Endothelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 95-100. DOI： 10.13422/j.cnki.syfjx.20180627.

摘要

目的：通过建立糖氧剥夺（OGD）诱导的人脑微血管内皮细胞（h-BMECs）损伤模型，观察桃红四物汤对细胞的保护作用及机制。方法：体外建立人脑微血管内皮细胞糖氧剥夺损伤模型，随机分为正常组、模型组、桃红四物汤高、中、低质量浓度组（0.8，0.4，0.2 g ·L-1）和阳性药尼莫地平组。噻唑蓝（MTT）比色法检测h-BMECs细胞活性，酶联免疫吸附法（ELISA）测定细胞上清液中乳酸脱氢酶（LDH）水平、细胞中丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性，采用蛋白免疫印迹法（Western blot）检测内皮细胞血管内皮生长因子（VEGF）和缺氧诱导因子-1α（HIF-1α）表达水平。结果：桃红四物汤可显著提高拟缺血损伤的人脑微血管内皮细胞活性，降低LDH漏出率（P<0.05，P<0.01），改善细胞形态，降低MDA水平（P<0.05），增强SOD活性（P<0.05，P<0.01）。增加内皮细胞VEGF和HIF-α蛋白的表达（P<0.05，P<0.01）。结论：桃红四物汤对拟缺血损伤人脑微血管内皮细胞具有保护作用，其机制与增强细胞抗氧化能力，通过HIF-α途径增强VEGF表达有关，显示中药组方可发挥整体调节的治疗优势。

Abstract

Objective: To investigate the mechanism that Taohong Siwutang (TST) protectshuman brain cerebral microvascular endothelial cells (h-BMECs) injury induced by oxygen-glucose deprivation (OGD). Method: Theh-BMECs injury models were induced by OGD

and then the cells were randomly divided into six groups:normal control group

model group

TST high

middle and low dose (0.8

0.4

0.2 g ·L-1) groups and nimodipine group.3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide(MTT)staining was employed to assay the activities of h-BMECs; the content of superoxide dismutase(SOD)

malonaldehyde(MDA)in cells

andlactate dehydrogenase (LDH)in cell supernatantwere determined by enzyme-linked immunosorbent assay (ELISA); and the expression levels of hypoxia inducible factor-1α (HIF-α) andvascular endothelial growth factor (VEGF)were detected by Western blot. Result: TST could significantly improve the activities of mimic ischemia injured hMBECs

decrease LDH leakage rate

improve the morphology of h-MBECs obviously

reduce MDA level

enhance SOD activity

and up-regulate the protein expression levels of VEGF and HIF-α. Conclusion: TST has protective effects on OGD-injured h-BMECs

and its mechanism may be related to enhancing the antioxidant capacity

and up-regulating the expression of VEGF and HIF-α

showing the advantages of compound Chinese medicine in integrative regulation.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Qingpeng Past on Synovium Angiogenesis in Rats with Adjuvant Arthritis

Effects of Tongxinluo Submicron Powder Combined with Jinlida Granules on Akt/mTOR Signal Transduction Pathway in Rats with Diabetic Nephropathy

Expression of HIF-1α and VEGF in Airway Remodeling of Asthmatic Rats and Effection by Administrated Shegan Mahuang Tang

Related Author

LIU Xin

ZOU Zhong-lan

MEI Quan-hui

ZHANG Heng-ping

HUANG Yan

LI Hui-min

LI Bao-li

DING Ying-jun

Related Institution

First Affiliated Hospital of University of South China

Deapartment Clinical of University of South China

Chang De city the first people’s hospital

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰