Effect of Xugudan on Expression of VEGF and TGF- in Rats with Nonunion of Tibia

LI Yue-wei; ZHOU Wei; GUO Jia-bao; SONG Ya-fang; WANG Xu-dong

doi:10.13422/j.cnki.syfjx.20180628

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Effect of Xugudan on Expression of VEGF and TGF- in Rats with Nonunion of Tibia

更新时间：2024-01-04

- Effect of Xugudan on Expression of VEGF and TGF- in Rats with Nonunion of Tibia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 8, Pages: 91-96(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.20180628
  CLC： R285.5
- Published：2018
- 稿件说明：
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LI Yue-wei, ZHOU Wei, GUO Jia-bao, et al. Effect of Xugudan on Expression of VEGF and TGF- in Rats with Nonunion of Tibia[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 91-96.
DOI：

LI Yue-wei, ZHOU Wei, GUO Jia-bao, et al. Effect of Xugudan on Expression of VEGF and TGF- in Rats with Nonunion of Tibia[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 91-96. DOI： 10.13422/j.cnki.syfjx.20180628.

摘要

目的：研究续骨丹外敷疗法对大鼠胫骨不连模型血管内皮生长因子（vascular endothelial growth factor，VEGF）及转化生长因子-β（transforming growth factor-β，TGF-β）表达的影响。方法：选取10只SD大鼠为空白组，成模50只SD大鼠随机分为模型组，续骨丹高、中、低剂量组（150，75，25 mg·kg-1），经皮电刺激神经组（transcutaneous electrical nerve stimulation，TENS）。分别在干预后4，8周对大鼠行X射线检测、测量骨间距；苏木素-伊红（HE）染色观察组织形态变化；实时荧光定量PCR（Real-time PCR）和酶联免疫吸附法（enzyme linked immunosorbent assay，ELISA）分别检测骨损伤区和血清中VEGF，TGF-β变化。结果：与模型组比较，续骨丹高、中、低剂量组，TENS组骨间距和VEGF，TGF-β表达显著升高（P<0.01）；与TENS组比较，续骨丹高剂量组骨间距和VEGF，TGF-β表达显著升高（P<0.01），续骨丹中剂量组差异不显著，续骨丹低剂量组显著降低（P<0.05，P<0.01）。结论：续骨丹可以通过调节VEGF，TGF-β的表达，改善局部血流供应，诱导血管重建和骨重建，促进骨不连愈合。

Abstract

Objective: To observe the effect ofexternal application of Xugudan on expression of vascular endothelial growth factor(VEGF) and transforming growth factor-β (TGF-β) in rat model of tibial nonunion. Method: A total of 10 SD rats were selected as blank group

and 50 modeled SD rats were randomly divided into model group

high

middle and low dose Xugudan(150

25 mg · kg-1) groups

and transcutaneous electrical nerve stimulation (TENS) group. In 4 weeks

and 8 weeks after the intervention

X-ray detection and measurement of bone spacing were conducted; hematoxylin eosin (HE) staining was used to observe the morphological changes. Real-time PCR and enzyme linked immunosorbent assay (ELISA) were used todetect VEGF

and TGF-β changes in bone injury and serum. Result: As compared with the model group

bone spacing

VEGF and TGF-β expression levels were significantly increased in Xugudan high

middle and low dose groups as well as TENS group(P<0.01). As compared with TENS group

bone spacing

VEGF and TGF-β expression levels were significantly increased in Xugudan high dose group (P<0.01)

butthe difference was not significant from Xugudan middle dose group

and the levels were significantly decreased in Xugudan low dose group (P<0.01

P<0.05). Conclusion: Xugudan can improve the local blood supply by regulating the expression of VEGF and TGF-β

induce vascular remodeling and bone remodeling

and promote the nonunion healing.

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