Effects of Danshen Injection on Expression of Hypoxia Inducible Factor-1 and Vascular Endothelial Growth Factor in Rats with Spinal Cord Injury

LIU Yang; GAO Yu-ting; MIAO Yu-chuan

doi:10.13422/j.cnki.syfjx.20180629

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Effects of Danshen Injection on Expression of Hypoxia Inducible Factor-1 and Vascular Endothelial Growth Factor in Rats with Spinal Cord Injury

更新时间：2024-01-04

- Effects of Danshen Injection on Expression of Hypoxia Inducible Factor-1 and Vascular Endothelial Growth Factor in Rats with Spinal Cord Injury
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 8, Pages: 110-115(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.20180629
  CLC： R285.5
- Published：2018
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LIU Yang, GAO Yu-ting, MIAO Yu-chuan. Effects of Danshen Injection on Expression of Hypoxia Inducible Factor-1 and Vascular Endothelial Growth Factor in Rats with Spinal Cord Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 110-115.
DOI：

LIU Yang, GAO Yu-ting, MIAO Yu-chuan. Effects of Danshen Injection on Expression of Hypoxia Inducible Factor-1 and Vascular Endothelial Growth Factor in Rats with Spinal Cord Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 110-115. DOI： 10.13422/j.cnki.syfjx.20180629.

摘要

目的：观察丹参注射液对大鼠脊髓损伤（SCI）后低氧诱导因子（HIF）-1α和血管内皮生长因子（VEGF）表达的影响，探讨其参与脊髓损伤修复的机制。方法：选取50只SD大鼠（SPF级）建立Allens'重力打击SCI模型（正常组除外）后，随机分为正常组、模型组、丹参治疗组、丹参加雷帕霉素组和甲泼尼龙琥珀酸钠组（每组10只）。正常组常规饲养，其余实验组均按1 mL·kg-1·d-1剂量分别腹腔注射生理盐水、丹参注射液、丹参注射液（含雷帕霉素3 mg·kg-1）和尾静脉注射甲泼尼龙琥珀酸钠溶液30 g·L-1。伤后1，3，7，14 d时采用联合行为评分法（CBS）评价大鼠脊髓神经功能恢复情况。伤后14 d处死动物，采用免疫组化染色和蛋白免疫印迹法（Western blot）检测各实验组HIF-1α和VEGF的表达差异。结果：SCI后14 d，与正常组比较，模型组大鼠CBS评分升高，HIF-1α和VEGF表达升高（P < 0.05）；与模型组比较，丹参治疗组CBS评分降低，HIF-1α和VEGF表达升高（P < 0.05）；与丹参治疗组比较，丹参加雷帕霉素组CBS评分升高，而HIF-1α和VEGF表达降低（P < 0.05）；上述指标在丹参治疗组和甲泼尼龙琥珀酸钠组之间的差异不具有统计学意义。结论：丹参注射液可通过调节HIF-1α和VEGF的表达从而参与脊髓的损伤修复。

Abstract

Objective: To explore the mechanism of Danshen injection on promoting spinal cord functional recovery by observing the expression of hypoxia inducible factor (HIF)-1α and vascular endothelial growth factor(VEGF) in rats with spinal cord injury(SCI). Method: Fifty SD rats were selected and divided randomly into normal control group

model group

Danshen injection treatment group

Danshen injection+rapamycin group and methylprednisolone sodium group(n=10 in each group). The rats in normal group were fed normally

and all the rats in other groups received 1 mL · kg-1 · d-1 normal saline

Danshen injection

Danshen injection (including rapamycin 3 mg · kg-1) by intraperitoneal injection and methylprednisolone sodium solution by tail vein injection.The combine behavioral score(CBS) was used on 1

14 d after SCI to evaluate the recovery of spinal nerve function in rats. All theses rats were killed on 14 d after SCI

and the expression levels of HIF-1α and VEGF were detected with immunohistochemical staining method and Western blot technique. Result: 14 d after SCI

as compared with the normal control group

the CBS and expression levels of HIF-1α and VEGF were elevated in the model group (P<0.05). As compared with the model group

the CBS was declined and the expression levels of HIF-1α and VEGF were elevated in Danshen injection treatment group (P<0.05). As compared with the Danshen injection treatment group

the CBS was elevated and the expression levels of HIF-1α and VEGF were declined in Danshen injection+rapamycin group(P<0.05). The differences of these indicators were not statistically significant between Danshen injection treatment group and methylprednisolone sodium group. Conclusion: Danshen injection could promote the recovery of nerve function after SCI by regulating the expression of HIF-1α and VEGF.

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