Objective: To observe the effect of Jianpi Xiaoai prescription and its decomposed recipes on the body weight

tumors

metastasis rate and expressions of Caspase-3

B-cell lymphoma/leukemia-xL (Bcl-xL) and Bcl-2 associated X protein (Bax) in colorectal cancer model nude mice.Method: The colorectal cancer model was established

and divided into control group

the Jianpi Yiqi group (15 g · kg-1)

the Huayu Jiedu group (15 g · kg-1)

the Jianpi Xiaoai prescription group (omni-recipe group

15 g · kg-1) and the 5-fluorouracil group (5-Fu

20 mg · kg-1). The body weight

tumor inhibition rate and metastasis rate were observed

and the protein expressions of Caspase-3

Bcl-xL

and Bax of nude mice were detected by Western blot after the corresponding drug intervention for 4 weeks.Result: The weight loss of the Jianpi Yiqi group and the omni-recipe group was less than that of control group (P<0.05)

Huayu Jiedu group' weight loss was higher than that of control group (P<0.05). The Jianpi Yiqi group had no statistical differences from control group in tumor inhibition rate; the Huayu Jiedu group and the omni-recipe group had a better tumor inhibition rate (P<0.05). All of the groups had different degrees of metastasis. Specifically

the control group and the Jianpi Yiqi group had the overall metastasis rate of 90%

and the Huayu Jiedu group and the omni-recipe group had the metastasis rate of 70% and 60%

respectively

both of which were lower than that of control group (P<0.05). Compared with control group

the Huayu Jiedu group and the omni-recipe group showed increase in the protein expression of Bax

and decrease in the protein expressions of Caspase-3

Bcl-xL (P<0.01).Conclusion: The Jianpi Yiqi group shows a stable weight

while the Huayu Jiedu group shows inhibitions in tumor and metastasis. The omni-recipe group shows a stable weight and inhibitions in tumor and metastasis

with the best effect. The antitumor mechanism of Jianpi Xiaoai prescription may be correlated with the up-regulation in expressions of apoptosis-associated proteins Bax and the down-regulation in Caspase-3

Bcl-xL induced apoptosis of tumor cells.