Objective: To observe the effect of Jianpi Xiaoai prescription (JPXAD) on the transforming growth factor-β (TGF-β)/long noncoding RNA-ATB (lncRNA-ATB)/microRNA 200a (miR-200a) signal pathway in colon cancer cells SW620

in order to study the anti-metastatic effect of JPXAD in colorectal cancer.Method: The 10%

15%

20% JPXAD-containing serum was given to SW620 cells after the treatment with TGF-β

Real-time PCR was applied to detect the mRNA expressions of lncRNA-ATB

miR-200a and Zinc finger E-box-binding homeobox 1(ZEB1)

and Western blot was used to determine the protein expression of ZEB1.Result: Compared with the control group

the relative expression of lncRNA-ATB in the TGF-β group increased

while the relative expression of miR-200a decreased

and the relative mRNA expression of ZEB1 increased significantly (P<0.01); compared with the TGF-β group

the expression level of lncRNA-ATB in low-dose JPXAD group decreased

while miR-200a increased

and ZEB1 decreased significantly (P<0.05); compared with the TGF-β group

the expression level of lncRNA-ATB in median-and high-dose JPXAD groups decreased

while miR-200a increased

and ZEB1 decreased significantly (P<0.01). Compared with control group

the relative protein expression of ZEB1 in the TGF-β group increased significantly (P<0.05); compared with the TGF-β group

the protein expression of ZEB1 in low-dose JPXAD group decreased slightly; compared with the TGF-β group

the protein expression of ZEB1 in median-and high-dose JPXAD groups decreased significantly (P<0.01).Conclusion: JPXAD may inhibit the metastasis of colorectal cancer by inhibiting the expression of lncRNA-ATB induced by TGF-β

increasing the expression of miR-200 and decreasing the protein expression of ZEB1.