Effect of Polyphyllin Ⅰ on Apoptosis and Protein Expressions of Bax, Bcl-2 and Caspase-3 in Colorectal Cancer Cell HCT116

LUO Ji; LUO Yan; LI Yong-min; TAN Xiao-ning; LYU Yuan; MA Rong-li; JIANG Yi-lan

doi:10.13422/j.cnki.syfjx.20180687

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Effect of Polyphyllin Ⅰ on Apoptosis and Protein Expressions of Bax, Bcl-2 and Caspase-3 in Colorectal Cancer Cell HCT116

更新时间：2024-01-04

- Effect of Polyphyllin Ⅰ on Apoptosis and Protein Expressions of Bax, Bcl-2 and Caspase-3 in Colorectal Cancer Cell HCT116
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 6, Pages: 172-176(2018)
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- DOI：10.13422/j.cnki.syfjx.20180687
  CLC： R285
- Published：2018
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LUO Ji, LUO Yan, LI Yong-min, et al. Effect of Polyphyllin Ⅰ on Apoptosis and Protein Expressions of Bax, Bcl-2 and Caspase-3 in Colorectal Cancer Cell HCT116[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 172-176.
DOI：

LUO Ji, LUO Yan, LI Yong-min, et al. Effect of Polyphyllin Ⅰ on Apoptosis and Protein Expressions of Bax, Bcl-2 and Caspase-3 in Colorectal Cancer Cell HCT116[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 172-176. DOI： 10.13422/j.cnki.syfjx.20180687.

摘要

目的：观察重楼皂苷Ⅰ（polyphyllin Ⅰ，PPⅠ）对结肠癌HCT116细胞凋亡及凋亡相关因子Bcl-2相关X蛋白（Bcl-2 associated X protein，Bax），B细胞淋巴瘤-2（B cell lymphoma-2，Bcl-2），半胱天冬酶-3（Caspase-3）蛋白表达的影响，探讨重楼皂苷Ⅰ抑制结直肠癌转移的可能作用机制。方法： Cell counting kit-8（CCK-8）法检测12，24，48 h的细胞生长抑制作用，流式细胞术检测不同浓度的重楼皂苷Ⅰ对HCT116细胞凋亡的影响，蛋白免疫印迹法（Western blot）检测凋亡相关因子Bax，Bcl-2，Caspase-3蛋白的表达。结果：重楼皂苷Ⅰ可抑制对HCT116细胞生长，呈一定的浓度、时间依赖性；可促进HCT116细胞凋亡，呈一定的浓度依赖性。与空白组、重楼皂苷Ⅰ低浓度组比较，重楼皂苷Ⅰ高浓度组Bax，剪切的半胱天冬酶-3蛋白（cleaved Caspase-3）蛋白表达升高，Bcl-2蛋白表达降低（P<0.05），未剪切的半胱天冬酶-3（pro-Caspase-3）蛋白表达无明显变化。结论：重楼皂苷Ⅰ可抑制HCT116细胞生长，诱导HCT116细胞凋亡，且其诱导HCT116细胞凋亡的机制可能与其降低线粒体途径相关的细胞凋亡因子Bcl-2表达，升高Bax表达，并上调线粒体通路下游的Caspase-3蛋白相关。

Abstract

Objective: To observe the effect of polyphyllin Ⅰ on the protein expression of Caspase-3 in the apoptosis of colon cancer HCT116 cells and apoptosis-related protein Bcl-2 associated X protein (Bax) and B cell lymphoma-2 (Bcl-2)

in order to investigate the inhibitory effect of polyphyllin Ⅰ on the metastasis of colorectal cancer.Method: Cell counting kit-8(CCK-8) experiment was performed for the detection of the cell growth inhibition effect; flow cytometry was used to detect the apoptosis rate of HCT116 cells after the treatment with polyphyllin Ⅰ; and Western blot was used to detect the expression of apoptosis-related proteins (Bax

Bcl-2 and Caspase-3).Result: Polyphyllin Ⅰ can inhibit the growth of HCT116 cells in a concentration and time-dependent manner

and promote the apoptosis of HCT116 cells in a concentration-dependent manner. Compared with control group and the low-concentration polyphyllin Ⅰ group

the expressions of Bax and cleaved Caspase-3 were increased in the high-concentration polyphyllin Ⅰ group

and the protein expression of Bcl-2 was decreased (P<0.05).Conclusion: Polyphyllin Ⅰ can inhibit the growth of HCT116 cells and promote the apoptosis of HCT116 cells. Its mechanism in promoting HCT116 cell apoptosis may be related to the decrease of the expression of the mitochondrial pathway-associated apoptosis factor Bcl-2

increase of the protein expressions of Bax and mitochondrial pathway downstream Caspase-3.

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