Objective: To establish the fingerprint of one year old

two years old Hedysari Radix and Astragalus Radix from Gansu province

in order to compare the similarity between them.Method: By HPLC-ELSD

Agilent ZROBAX-SBC18 (4.6 mm×250 mm

5 μm) chromatographic column was adopted

with A water and B acetonitrile as the mobile phase

and eluted in a gradient mode. The column temperature was set at 35℃

and the sample volume was 15 μL. Evaporative light detector was Alltech ELSD 2000

the drift tube temperature was 110℃

carrier gas flow rate was 3.0 L · min-1.The fingerprint was established with 24 batches of Hedysari Radix and Astragalus Radix respectively.Result: The common peaks of one year old and two years old Hedysari Radix were 8 and 7

respectively

and the similarities were 0.923 and 0.825

respectively. There were six same peaks

which were respectively calycosin-7-glucoside

ononin

soybean saponin I

formononetin

isoastragaloside Ⅱ and astragaloside I. The common peaks of one year old and two years old Astragalus Radix were 25 and 17

respectively

and the similarities were 0.980 and 0.997

respectively. There were seven same peaks

which were calycosin-7-glucoside

ononin

calycosin

astragaloside Ⅳ

astragaloside Ⅲ

isoastragaloside Ⅱ and astragaloside I. The similarity was 0.032 between one year old Hedysari Radix and Astragalus Radix

and was 0.023 between two years old Hedysari Radix and Astragalus Radix. There were four common peaks

namely calycosin-7-glucoside

ononin and isoastragaloside Ⅱ and astragaloside I.Conclusion: The differences between one year old

two years old Hedysari Radix and Astragalus Radix medicinal herbs were obvious

and the two drugs could be effectively differentiated. There are a few common components between them

with a low similarity and great quality differences. Therefore

it is not suitable to substitute Hedysari Radix and Astragalus Radix with each other.